VEGFR1/Flt-1 Antibody (EWF) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1006

Novus Biologicals, part of Bio-Techne
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NB600-1006
NB600-1006-0.02mg
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Validated:

CyTOF-ready, ELISA, Flow Cytometry, Immunoprecipitation, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # EWF

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all VEGFR1/Flt-1 Antibodies »

Product Specifications

Immunogen

Recombinant human soluble Flt-1 (D5)

Reactivity Notes

Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Type I membrane protein (Flt1) and soluble (SFlt1).

Specificity

This will recognise the recombinant and naturally occurring form of human VEGFR1/Flt1.

Marker

Endothelial Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

PLEASE NOTE: 0.02mg size is provided in liquid format.

Scientific Data Images for VEGFR1/Flt-1 Antibody (EWF) - Azide and BSA Free

Western Blot: VEGFR1/Flt-1 Antibody (EWF)Azide and BSA Free [NB600-1006]

Western Blot: VEGFR1/Flt-1 Antibody (EWF)Azide and BSA Free [NB600-1006]

Western Blot: VEGFR1/Flt-1 Antibody (EWF) [NB600-1006] - VEGF Receptor 1 Antibody (EWF) [NB600-1006] - The monoclonal anti-human VEGFR-1/Flt-1 cross reacts with recombinant murine sFlt-1.
Flow Cytometry: VEGFR1/Flt-1 Antibody (EWF) - Azide and BSA Free [NB600-1006]

Flow Cytometry: VEGFR1/Flt-1 Antibody (EWF) - Azide and BSA Free [NB600-1006]

Flow Cytometry: VEGFR1/Flt-1 Antibody (EWF) [NB600-1006] - analysis with porcine aortic endothelial cells expressing human VEGFR-1/Flt-1.

Applications for VEGFR1/Flt-1 Antibody (EWF) - Azide and BSA Free

Application
Recommended Usage

ELISA

1-10 ug/ml

Immunoprecipitation

1-5 ug/ml lysate

Western Blot

1-10 ug/ml
Application Notes
WB: Predicted molecular weight: 150 kDa. Optimal dilutions/concentrations should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VEGFR1/Flt-1

Receptors for VEGF, VEGFB and PGF have tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. VEGF and its high-affinity binding receptors--the tyrosine kinases FLK1 and FLT1--are thought to be important for the development of embryonic vasculature. It has been shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis.

Long Name

Vascular Endothelial Growth Factor Receptor 1

Alternate Names

Flt-1, FLT1, FRT, VEGF R1, VEGFR-1

Entrez Gene IDs

2321 (Human)

Gene Symbol

FLT1

UniProt

P17948

Additional VEGFR1/Flt-1 Products

Product Documents for VEGFR1/Flt-1 Antibody (EWF) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VEGFR1/Flt-1 Antibody (EWF) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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