VEGFR3/Flt-4 Antibody (AFL4) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43259
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Dual RNAscope ISH-IHC, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Cited:
Dual RNAscope ISH-IHC, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2a Kappa Clone # AFL4
Format
BSA Free
Concentration
0.5 mg/ml
Product Specifications
Immunogen
The immunogen for this antibody was VEGFR3.
Reactivity Notes
This antibody is reactive to Mouse.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2a Kappa
Scientific Data Images for VEGFR3/Flt-4 Antibody (AFL4) - BSA Free
Immunohistochemistry-Frozen: VEGFR3/Flt-4 Antibody (AFL4) - BSA Free [NBP1-43259]
Immunohistochemistry-Frozen: VEGFR3/Flt-4 Antibody (AFL4) [NBP1-43259] - VEGFR-3 (green) was detected in murine cancer using VEGFR-3-AlexaFluor488 antibody (1:50; 1 hour) in PBS. Nuclei were stained with DAPI (blue). Tissue was fixed in acetone. Image from a verified customer review using the Alexa Fluor488 version of this VEGFR-3 antibody.Flow Cytometry: VEGFR3/Flt-4 Antibody (AFL4) - BSA Free [NBP1-43259]
Flow Cytometry: VEGFR3/Flt-4 Antibody (AFL4) [NBP1-43259] - Surface staining of MC/9 cell line with Anti-Mouse Fc epsilon Receptor I alpha (Fc epsilon R1) FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.Applications for VEGFR3/Flt-4 Antibody (AFL4) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunohistochemistry
1:10-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
The AFL4 antibody has been tested by flow cytometric analysis of in vitro differentiated mouse endothelial cells. In brief, mouse ES cells were incubated on collagen IV matrix for 4 days and subsequently stimulated with VEGF under serum free conditions to induce further differentiation. Dual ISH-IHC validation (PMID: 31083674).
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: VEGFR3/Flt-4
Long Name
Vascular Endothelial Growth Factor Receptor 3
Alternate Names
Flt-4, FLT4, VEGF R3
Gene Symbol
FLT4
Additional VEGFR3/Flt-4 Products
Product Documents for VEGFR3/Flt-4 Antibody (AFL4) - BSA Free
Product Specific Notices for VEGFR3/Flt-4 Antibody (AFL4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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