Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S187-7

Concentration

1 mg/ml

View all VGCNL1 Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 1659-1738 (cytoplasmic C-terminus) of rat NALCN

Localization

Membrane

Specificity

Detects approx 200kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for VGCNL1 Antibody (S187-7)

Western Blot: VGCNL1 Antibody (S187-7) [NBP2-22410]

Western Blot: VGCNL1 Antibody (S187-7) [NBP2-22410]

Western Blot: VGCNL1 Antibody (S187-7) [NBP2-22410] - Western Blot analysis of Rat Brain showing detection of ~200 kDa VGCNL1protein using Mouse Anti-VGCNL1Monoclonal Antibody, Clone S187-7 (NBP2-22410). Lane 1: Molecular Weight (MW) Ladder. Lane 3: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-VGCNL1Monoclonal Antibody (NBP2-22410) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~200 kDa.
Immunocytochemistry/ Immunofluorescence: VGCNL1 Antibody (S187-7) [NBP2-22410]

Immunocytochemistry/ Immunofluorescence: VGCNL1 Antibody (S187-7) [NBP2-22410]

Immunocytochemistry/Immunofluorescence: VGCNL1 Antibody (S187-7) [NBP2-22410] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGCNL1Monoclonal Antibody, Clone S187-7 (NBP2-22410). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VGCNL1Monoclonal Antibody (NBP2-22410) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VGCNL1Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: VGCNL1 Antibody (S187-7) [NBP2-22410]

Immunocytochemistry/ Immunofluorescence: VGCNL1 Antibody (S187-7) [NBP2-22410]

Immunocytochemistry/Immunofluorescence: VGCNL1 Antibody (S187-7) [NBP2-22410] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NALCN Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X.
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Applications for VGCNL1 Antibody (S187-7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
1 ug/ml of NALCN Antibody was sufficient for detection of NALCN in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP asthe secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VGCNL1

NALCN forms a voltage-independent, nonselective, noninactivating cation channel permeable to Na+, K+, and Ca(2+). It isresponsible for the neuronal background sodium leak conductance (Lu et al., 2007 (PubMed 17448995)).(supplied by OMIM)

Alternate Names

bA430M15.1, CanIonFLJ44659, FLJ23913, FLJ44764, four repeat voltage-gated ion channel, MGC74524, sodium leak channel non-selective protein, sodium leak channel, non-selective, VGCNL1, voltage gated channel like 1, Voltage gated channel-like protein 1

Gene Symbol

NALCN

UniProt

Q6Q760 (Rat)

Additional VGCNL1 Products

Product Documents for VGCNL1 Antibody (S187-7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VGCNL1 Antibody (S187-7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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