VGF Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31596

Novus Biologicals, part of Bio-Techne
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NBP2-31596
NBP2-31596-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VGF Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: APARDELPDWNEVLPPWDREEDEVYPPGPYHPFPNYIRPRTLQPPSALRRRHYHHALPPSRHYPGREAQARRAQEEAEAEERRLQEQEELENYIEHV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VGF Antibody

Immunocytochemistry/ Immunofluorescence: VGF Antibody [NBP2-31596]

Immunocytochemistry/ Immunofluorescence: VGF Antibody [NBP2-31596]

Immunocytochemistry/Immunofluorescence: VGF Antibody [NBP2-31596] - Staining of human cell line SK-MEL-30 shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596] - Staining of human prostate shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596] - Staining of human cerebral cortex shows high expression.
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Applications for VGF Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VGF

Nerve growth factor (NGF) is a peptide that plays a key role in the differentiation and survival of neurons in the peripheral nervous system (PNS) and the central nervous system (CNS). VGF is a peptide synthesized and secreted by neurons and is upregulated by NGF in the PC12 cell line. VGF is widely expressed in both the PNS and CNS, but is especially abundant in the adult hypothalamus. VGF plays an essential role in how the brain regulates energy expenditure and body weight. Its expression is rapidly induced by injury, the circadian clock, and neuronal activity.

Long Name

Neurosecretory Protein VGF

Alternate Names

neuro-endocrine specific protein VGF, neurosecretory protein VGF, VGF nerve growth factor inducible

Gene Symbol

VGF

UniProt

O15240

Additional VGF Products

Product Documents for VGF Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VGF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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