Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S28-9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all VGLUT1/SLC17A7 Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1

Localization

Cytoplasmic Vesicle , Secretory Vesicle , Synaptic Vesicle Membrane , Membrane , Cell Junction , Synapse

Specificity

Detects 52kDa. No cross-reactivity against VGlut2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for VGLUT1 Antibody (S28-9)

Western Blot: VGLUT1 Antibody (S28-9) [NBP2-59329]

Western Blot: VGLUT1 Antibody (S28-9) [NBP2-59329]

Western Blot: VGLUT1 Antibody (S28-9) [NBP2-59329] - Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT1 protein using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (NBP2-59329). Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody (NBP2-59329) at 1:1000.
Immunocytochemistry/ Immunofluorescence: VGLUT1 Antibody (S28-9) [NBP2-59329]

Immunocytochemistry/ Immunofluorescence: VGLUT1 Antibody (S28-9) [NBP2-59329]

Immunocytochemistry/Immunofluorescence: VGLUT1 Antibody (S28-9) [NBP2-59329] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (NBP2-59329). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody (NBP2-59329) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VGLUT1 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: VGLUT1 Antibody (S28-9) [NBP2-59329]

Immunocytochemistry/ Immunofluorescence: VGLUT1 Antibody (S28-9) [NBP2-59329]

Immunocytochemistry/Immunofluorescence: VGLUT1 Antibody (S28-9) [NBP2-59329] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (NBP2-59329). Tissue: Differentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody (NBP2-59329) at 1:100. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) VGLUT1 Antibody. (D) Hoechst (E) Composite.

Applications for VGLUT1 Antibody (S28-9)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: VGLUT1/SLC17A7

VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate.

Long Name

Vesicular Glutamate Transporter 1

Alternate Names

BNPI, SLC17A7

Gene Symbol

SLC17A7

Additional VGLUT1/SLC17A7 Products

Product Documents for VGLUT1 Antibody (S28-9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VGLUT1 Antibody (S28-9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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