VGLUT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13165

Novus Biologicals, part of Bio-Techne
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NBP3-13165
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VGLUT1/SLC17A7 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of mouse VGLUT1. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for VGLUT1 Antibody

Western Blot: VGLUT1 Antibody [NBP3-13165]

Western Blot: VGLUT1 Antibody [NBP3-13165]

Western Blot: VGLUT1 Antibody [NBP3-13165] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VGluT1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: VGLUT1 Antibody [NBP3-13165]

Immunocytochemistry/ Immunofluorescence: VGLUT1 Antibody [NBP3-13165]

Immunocytochemistry/Immunofluorescence: VGLUT1 Antibody [NBP3-13165] - VGLUT1 antibody detects VGLUT1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VGLUT1 stained by VGLUT1 antibody (NBP3-13165) diluted at 1:500. Red: Tau, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-13165]

Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-13165]

Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-13165] - Rat brain. VGLUT1 stained by VGLUT1 antibody (NBP3-13165) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Applications for VGLUT1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VGLUT1/SLC17A7

VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate.

Long Name

Vesicular Glutamate Transporter 1

Alternate Names

BNPI, SLC17A7

Gene Symbol

SLC17A7

Additional VGLUT1/SLC17A7 Products

Product Documents for VGLUT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VGLUT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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