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VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25743

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25743

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Rhesus Macaque (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1616

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free

Immunogen

Recombinant protein encompassing a sequence within the center region of human VIAAT/SLC32A1/VGAT. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus (80%).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free

VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free

Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free [NBP3-25743] -

Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free [NBP3-25743] - VGAT antibody [HL1616] detects VGAT protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: VGAT stained by VGAT antibody [HL1616] (NBP3-25743) diluted at 1:100. Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free

Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free [NBP3-25743] -

Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free [NBP3-25743] - VGAT antibody [HL1616] detects VGAT protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Green: VGAT stained by VGAT antibody [HL1616] (NBP3-25743) diluted at 1:100. Red: NF-H, a Cytoplasm marker, stained by NF-H antibody [GT114] diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free [NBP3-25743] -

Immunocytochemistry/Immunofluorescence: VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free [NBP3-25743] - VGAT antibody [HL1616] detects VGAT protein at nucleus and cytoskeleton by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VGAT stained by VGAT antibody [HL1616] (NBP3-25743) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .

Applications for VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VIAAT/SLC32A1

The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles(McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Acid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).

Long Name

Vesicular Inhibitory Amino Acid Transporter

Alternate Names

SLC32A1, VGAT

Gene Symbol

SLC32A1

Additional VIAAT/SLC32A1 Products

Product Documents for VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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