VIAAT/SLC32A1/VGAT Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-55238

Novus Biologicals, part of Bio-Techne
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NB110-55238-0.025ml
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse

Applications

Validated:

ELISA, Microarray, Western Blot

Cited:

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

4.3 mg/ml

View all VIAAT/SLC32A1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acids 1-20 located at the N-terminus of mouse VGAT.

Reactivity Notes

Rat, Mouse. This sequence is identical in rat VGAT and highly conserved (single a.a. substitution) in human VGAT.

Localization

Integral membrane protein. Intracellular membrane vesicles

Specificity

VGAT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications for VIAAT/SLC32A1/VGAT Antibody - BSA Free

Application
Recommended Usage

Western Blot

2-4 ug/ml
Application Notes
Use in ELISA reported in scientific literature (PMID 24772072)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

10mM PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

4.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VIAAT/SLC32A1

Synaptic transmission involves the regulated exocytosis of vesicles containing neurotransmitters. The inhibitory neurotransmitter g-aminobutyric acid (GABA) is synthesized in the cytoplasm from L-glutamate by the enzyme glutamic acid decarboxylase (GAD) and loaded in synaptic vesicles for exocytosis. The vesicular GABA transporter (VGAT, also termed vesicular inhibitory amino acid transporter or VIAAT, 52-57 kDa) is responsible for the uptake of GABA into synaptic vesicles, following synthesis by GAD, or reuptake by plasma membrane GABA transporters (GATs). VGAT protein (55 kDa) contains ten transmembrane domains and belongs to a new family of vesicular neurotransmitter transporters. VGAT is highly conserved in mouse, rat and human (95-98% identity). VGAT uses both chemical and electrical components of the luminal protein gradient to mediate the accumulation of GABA into synaptic vesicles. VGAT is also responsible for glycine uptake in glycinergic neurons. VGAT is a homolog of the c. elegans unc -47 protein involved in GABA transport. Unc-47 is expressed in GABA neurons, localizes to synaptic vesicles and confers vesicular GABA transport in transfected cells. Mutants of c. elegans in which the unc-47 gene encoding VGAT is non-functional or absent, exhibit a complete loss of GABAergic function and elevated levels of GABA in the cytoplasm. VGAT is present in nerve endings of inhibitory neurons containing GABA, but also in glycinergic neurons in the brain and retina.1,4,5 At subcellular levels, VGAT specifically associates with synaptic vesicles. VGAT/VIAAT is also expressed in rat pancreatic islet cells.

Long Name

Vesicular Inhibitory Amino Acid Transporter

Alternate Names

SLC32A1, VGAT

Entrez Gene IDs

140679 (Human); 22348 (Mouse); 83612 (Rat)

Gene Symbol

SLC32A1

UniProt

O35633 (Mouse)

Additional VIAAT/SLC32A1 Products

Product Documents for VIAAT/SLC32A1/VGAT Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for VIAAT/SLC32A1/VGAT Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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