Vinculin Antibody (9I3Y8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16145
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 9I3Y8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Vinculin Antibody (9I3Y8)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 800-900 of human Vinculin (NP_054706.1). DAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMM
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Vinculin Antibody (9I3Y8)
![Western Blot: Vinculin Antibody (9I3Y8) [NBP3-16145]](https://resources.bio-techne.com/images/products/Vinculin-Antibody-3J7P3-Western-Blot-NBP3-16145-img0002.jpg "Western Blot: Vinculin Antibody (9I3Y8) [NBP3-16145]")
Western Blot: Vinculin Antibody (9I3Y8) [NBP3-16145]
Western Blot: Vinculin Antibody (3J7P3) [NBP3-16145] - Western blot analysis of extracts of various cell lines, using Vinculin Rabbit mAb (NBP3-16145) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.![Immunohistochemistry-Paraffin: Vinculin Antibody (9I3Y8) [NBP3-16145]](https://resources.bio-techne.com/images/products/Vinculin-Antibody-3J7P3-Immunohistochemistry-Paraffin-NBP3-16145-img0001.jpg "Immunohistochemistry-Paraffin: Vinculin Antibody (9I3Y8) [NBP3-16145]")
Immunohistochemistry-Paraffin: Vinculin Antibody (9I3Y8) [NBP3-16145]
Immunohistochemistry-Paraffin: Vinculin Antibody (3J7P3) [NBP3-16145] - Immunohistochemistry of paraffin-embedded mouse skeletal muscle using Vinculin Rabbit mAb (NBP3-16145) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.![Immunoprecipitation: Vinculin Antibody (9I3Y8) [NBP3-16145]](https://resources.bio-techne.com/images/products/Vinculin-Antibody-3J7P3-Immunoprecipitation-NBP3-16145-img0003.jpg "Immunoprecipitation: Vinculin Antibody (9I3Y8) [NBP3-16145]")
Immunoprecipitation: Vinculin Antibody (9I3Y8) [NBP3-16145]
Immunoprecipitation: Vinculin Antibody (3J7P3) [NBP3-16145] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Vinculin antibody (NBP3-16145). Western blot was performed from the immunoprecipitate using Vinculin antibody (NBP3-16145) at a dilition of 1:1000.Applications for Vinculin Antibody (9I3Y8)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunoprecipitation
1:500 - 1:1000
Western Blot
1:10000 - 1:300000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Vinculin
Long Name
Metavinculin
Alternate Names
MVCL, VCL
Gene Symbol
VCL
Additional Vinculin Products
Product Documents for Vinculin Antibody (9I3Y8)
Product Specific Notices for Vinculin Antibody (9I3Y8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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