Vinculin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-55796

Novus Biologicals, part of Bio-Techne
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NBP2-55796
NBP2-55796-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Vinculin Antibodies »

Product Summary for Vinculin Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: SALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Vinculin Antibody

Western Blot: Vinculin Antibody [NBP2-55796]

Western Blot: Vinculin Antibody [NBP2-55796]

Western Blot: Vinculin Antibody [NBP2-55796] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: Vinculin Antibody [NBP2-55796]

Immunocytochemistry/ Immunofluorescence: Vinculin Antibody [NBP2-55796]

Immunocytochemistry/Immunofluorescence: Vinculin Antibody [NBP2-55796] - Staining of human cell line U-2 OS shows localization to focal adhesion sites. Antibody staining is shown in green.
Western Blot: Vinculin Antibody [NBP2-55796]

Western Blot: Vinculin Antibody [NBP2-55796]

Western Blot: Vinculin Antibody [NBP2-55796] - Analysis in human cell line PC-3 and human cell line MCF-7.

Applications for Vinculin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Vinculin

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. (provided by RefSeq)

Long Name

Metavinculin

Alternate Names

MVCL, VCL

Gene Symbol

VCL

Additional Vinculin Products

Product Documents for Vinculin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Vinculin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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