VIPR1/VPAC1 Antibody (PD00-96)

Western Blot: VIPR1/VPAC1 Antibody (PD00-96) [NBP3-33097]

Western Blot: VIPR1/VPAC1 Antibody (PD00-96) [NBP3-33097] - Western blot analysis of VIPR1/VPAC1 on different lysates with Rabbit anti-VIPR1/VPAC1 antibody (NBP3-33097) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 ug/Lane)
Lane 2: Jurkat cell lysate (20 ug/Lane)
Lane 3: Raji cell lysate (20 ug/Lane)
Lane 4: HT-29 cell lysate (20 ug/Lane)
Lane 5: Caco-2 cell lysate (20 ug/Lane)
Lane 6: MCF7 cell lysate (20 ug/Lane)
Lane 7: MDA-MB-231 cell lysate (20 ug/Lane)
Lane 8: PC-12 cell lysate (20 ug/Lane)
Lane 9: LNCaP cell lysate (20 ug/Lane)

Predicted band size: 52 kDa
Observed band size: 55 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33097) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: VIPR1/VPAC1 Antibody (PD00-96) [NBP3-33097]

Immunohistochemistry: VIPR1/VPAC1 Antibody (PD00-96) [NBP3-33097] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-VIPR1/VPAC1 antibody (NBP3-33097) at 1/4,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33097) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.