Viral Dengue Virus 1/3 NS1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9440
Key Product Details
Species Reactivity
Viral
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 969956
Product Specifications
Immunogen
Human embryonic kidney cell line HEK293-derived recombinant Dengue Virus 3 NS1 protein
Asp1-Ala352
Accession # YP_001531169
Asp1-Ala352
Accession # YP_001531169
Specificity
Detects recombinant Dengue Virus 1 NS1 protein and recombinant Dengue Virus 3 NS1 protein in direct ELISAs and Western blots. In direct ELISAs and Western Blots, no cross-reactivity with recombinant Dengue Virus 2 and 4 NS1 proteins was observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Viral Dengue Virus 1/3 NS1 Antibody
Detection of Dengue Virus 1/3 NS1 by Western Blot.
Western blot shows recombinant Dengue Virus (DENV) 1, recombinant DENV2, recombinant DENV3, and recombinant DENV4. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Viral Dengue Virus 1/3 NS1 Monoclonal Antibody (Catalog # MAB9440) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Dengue Virus 1/3 NS1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Viral Dengue Virus 1/3 NS1 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Recombinant Dengue Virus 1 and recombinant Dengue Virus 3
Sample: Recombinant Dengue Virus 1 and recombinant Dengue Virus 3
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Dengue Virus 1/3 NS1
Additional Dengue Virus 1/3 NS1 Products
Product Documents for Viral Dengue Virus 1/3 NS1 Antibody
Product Specific Notices for Viral Dengue Virus 1/3 NS1 Antibody
For research use only
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