Viral Dengue Virus 2/4 NS1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9439

R&D Systems, part of Bio-Techne
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MAB9439-100
MAB9439-SP
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Key Product Details

Species Reactivity

Viral

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 969803

View all Dengue Virus 2/4 NS1 Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell line HEK293-derived recombinant Dengue Virus 2 NS1 protein
Asp1-Ala352
Accession # NP_739584

Specificity

Detects recombinant Dengue Virus 2 NS1 protein and recombinant Dengue Virus 4 NS1 protein in Western blot and Direct Elisa. Detects recombinant Dengue Virus 1 and 3 NS1 proteins in Direct Elisa. 

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Viral Dengue Virus 2/4 NS1 Antibody

Detection of Dengue Virus 2/4 NS1 antibody by Western Blot.

Detection of Dengue Virus 2/4 NS1 by Western Blot.

Western blot shows recombinant Dengue Virus (DENV) 1, recombinant DENV2, recombinant DENV3, and recombinant DENV4. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Dengue Virus 2/4 NS1 Monoclonal Antibody (Catalog # MAB9439) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Dengue Virus 2/4 NS1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for Viral Dengue Virus 2/4 NS1 Antibody

Application
Recommended Usage

Western Blot

5 µg/mL
Sample: Recombinant Dengue Virus 2 NS1 and recombinant Dengue Virus 4 NS1

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dengue Virus 2/4 NS1

Dengue virus (DENV), the cause of Dengue fever, is a single positive-stranded RNA virus of the family Flaviviridae, genus Flavivirus. 5 closely related but antigenically distinct serotypes of the virus have been found: DENV-1, DENV-2, DENV-3, DENV-4 and DENV-5.  DENV has a genome of about 11,000 bases, encoding a single large polyprotein that is subsequently cleaved into several structural and non-structural peptides. The polyprotein is divided into three structural proteins, C, prM, E, seven non-structural proteins, NS1, NS2a, NS2b, NS3, NS4a, NS4b, NS5, and short non-coding regions on both the 5' and 3' ends. DENV nonstructural protein-1 (NS1) is a secreted glycoprotein that is absent from viral particles but accumulates in the supernatant and on the plasma membrane of cells during infection.

Long Name

Dengue Virus 2/4 Polyprotein

Entrez Gene IDs

1494449 (Viral)

Gene Symbol

POLY

UniProt

NP_739584

Additional Dengue Virus 2/4 NS1 Products

Product Documents for Viral Dengue Virus 2/4 NS1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Viral Dengue Virus 2/4 NS1 Antibody

For research use only

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