Skip to main content

Viral Dengue Virus NS1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB94442

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
MAB94442-100
MAB94442-SP

Key Product Details

Species Reactivity

Viral

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 971332

Product Specifications

Immunogen

Human embryonic kidney cell line HEK293-derived recombinant Dengue Virus 4 NS1 protein
Asp1-Ala352
Accession # NP_740318

Specificity

In sandwich immunoassays, Dengue Virus 1/2/3/4 NS1 is detected.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Viral Dengue Virus NS1 Antibody

Dengue Virus NS1 Antibody in ELISA Standard Curve.

Dengue Virus NS1 ELISA Standard Curve.

Recombinant Dengue Virus 1 NS1, Recombinant Dengue Virus 2 NS1, Recombinant Dengue Virus 3 NS1, or Recombinant Dengue Virus 4 NS1 protein was serially diluted 2-fold and captured by Mouse Anti-Dengue Virus NS1 Monoclonal Antibody (Catalog # MAB94442) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Dengue Virus NS1 Monoclonal Antibody (Catalog # MAB94441) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Viral Dengue Virus NS1 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Dengue Virus NS1 Monoclonal Antibody (Catalog # MAB94441).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
Loading...

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dengue Virus NS1

Dengue virus (DENV), the cause of Dengue fever, is a single positive-stranded RNA virus of the family Flaviviridae, genus Flavivirus. 5 closely related but antigenically distinct serotypes of the virus have been found: DENV-1, DENV-2, DENV-3, DENV-4 and DENV-5.  DENV has a genome of about 11,000 bases, encoding a single large polyprotein that is subsequently cleaved into several structural and non-structural peptides. The polyprotein is divided into three structural proteins, C, prM, E, seven non-structural proteins, NS1, NS2a, NS2b, NS3, NS4a, NS4b, NS5, and short non-coding regions on both the 5' and 3' ends. DENV nonstructural protein-1 (NS1) is a secreted glycoprotein that is absent from viral particles but accumulates in the supernatant and on the plasma membrane of cells during infection.

Long Name

Dengue Virus Nauru/West Pac/1974 Polyprotein

Alternate Names

Dengue NS1, Dengue NS1 protein, Dengue Virus

UniProt

Additional Dengue Virus NS1 Products

Product Documents for Viral Dengue Virus NS1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Viral Dengue Virus NS1 Antibody

For research use only

Loading...
Loading...
Loading...
Loading...