Viral EBOV GP Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9016

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB9016-100
MAB9016-SP
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Key Product Details

Species Reactivity

Validated:

Viral

Cited:

Human, Hamster, Primate

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2A Clone # 993408

View all EBOV GP Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant Viral EBOV GP
Ile33-Arg501
Accession # Q05320

Specificity

Detects EBOV GP in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Viral EBOV GP Antibody

Detection of EBOV GP antibody by Western Blot.

Detection of EBOV GP by Western Blot.

Western blot shows Recombinant EBOV GP. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Viral EBOV GP Monoclonal Antibody (Catalog # MAB9016) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for EBOV GP at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Viral EBOV GP Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Recombinant EBOV GP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EBOV GP

The GP glycoprotein encoded by the genome of Ebola family viruses is a critical molecule for the pathogenicity of Ebolavirus hemorrhagic viruses (1, 2). It is processed into distinct forms for virus capsule or cell surface presentation or release from virus infected cells. The GP precursor protein is cleaved by furin at a multibasic site to yield a 140 kDa N-terminal fragment (GP1) and a 26 kDa C-terminal fragment (GP2) which remain disulfide linked (3). GP1 is entirely extracellular while GP2 is a transmembrane protein (4). Heterodimers of GP1-GP2 can further associate into trimers (5). GP expressed on virus infected cells can be shed by TACE mediated cleavage, liberating a disulfide linked complex of soluble GP1 and truncated GP2 (4-6). GP binds to multiple C-type lectins on target cell surfaces, including CLEC10A/MGL, DC-SIGN, and DC-SIGNR (7-9). Following internalization, GP1 is cleaved by Cathepsin B and Cathepsin L and then interacts with Niemann-Pick C1 (NPC1) in the endosomal membrane (10-12).

References

  1. Yang, Z.-Y. et al. (2000) Nat. Med. 6:886.
  2. de La Vega, M.-A. et al. (2015) Viral Immunol. 28:3.
  3. Volchkov, V.E. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5762.
  4. Volchkov, V.E. et al. (1998) Virology 245:110.
  5. Sanchez, A. et al. (1998) J. Virol. 72:6442.
  6. Dolnik, O. et al. (2004) EMBO J. 23:2175.
  7. Takada, A. et al. (2004) J. Virol. 78:2943.
  8. Alvarez, C.P. et al. (2002) J. Virol. 76:6841.
  9. Simmons, G. et al. (2003) Virology 305:115.
  10. Schornberg, K. et al. (2006) J. Virol. 80:4174.
  11. Chandran, K. et al. (2005) Science 308:1643.
  12. Cote, M. et al. (2011) Nature 477:344.

Long Name

Ebola Virus Glycoprotein

Alternate Names

bovGP

Entrez Gene IDs

911829 (Viral)

Gene Symbol

GP

UniProt

Q05320

Additional EBOV GP Products

Product Documents for Viral EBOV GP Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Viral EBOV GP Antibody

For research use only

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