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Viral wnvNS1 Protein Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB29072

R&D Systems, part of Bio-Techne
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MAB29072-100
MAB29072-SP

Key Product Details

Species Reactivity

Viral

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 991501

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with recombinant Viral wnvNS1 Protein
Asp1-Ala352
Accession # NP_776015

Specificity

Detects viral wnvNS1 Protein in direct ELISAs. In direct ELISAs, no cross-reactivity with viral Dengue NS1 (serotypes 1,2 and 3) and viral Zika NS1 proteins is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Viral wnvNS1 Protein Antibody

Detection of West Nile Virus (wnv) NS1 Protein antibody by Western Blot.

Detection of West Nile Virus (wnv) NS1 Protein by Western Blot.

Western blot shows recombinant West Nile Virus (wnv) NS1 protein. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Viral wnvNS1 Protein Monoclonal Antibody (Catalog # MAB29072) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for wnvNS1 Protein at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Viral wnvNS1 Protein Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Recombinant West Nile Virus (wnv) NS1 protein

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: wnvNS1 Protein

West Nile Virus, or WNV, is a member of the Flaviviridae family that includes other human pathogens such as yellow fever (YF), dengue (DEN), tick-borne encephalitis (TBE), Japanese encephalitis (JEV), and Murray Valley encephalitis (MVE) viruses. West Nile virus (WNV) is a single-stranded RNA mosquito-transmitted virus that causes epidemics of encephalitis and viscerotropic disease. The WNV nonstructural protein NS1 is a 48-kDa highly conserved secreted glycoprotein that does not package with the virion. Even when absent from the virion, immunization with NS1 typically elicits a protective immune response.

Long Name

West Nile Virus Non-structural Protein NS1

Entrez Gene IDs

912267 (Viral)

Gene Symbol

POLY

UniProt

Additional wnvNS1 Protein Products

Product Documents for Viral wnvNS1 Protein Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Viral wnvNS1 Protein Antibody

For research use only

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