Viral wnvNS3 Protease Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB29071
Key Product Details
Species Reactivity
Validated:
Viral
Cited:
Human, Mouse
Applications
Validated:
Immunoprecipitation, Neutralization, Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG3 Clone # 345712
Product Specifications
Immunogen
E. coli-derived recombinant wnvNS3 Protease protein
Ser1423-Lys1470, Gly1506-Leu1689
Accession # ABD85079
Ser1423-Lys1470, Gly1506-Leu1689
Accession # ABD85079
Specificity
Detects West Nile NS3 Protease in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3
Scientific Data Images for Viral wnvNS3 Protease Antibody
Detection of wnvNS3 Protease by Western Blot.
Western blot shows lysates of Recombinant Viral wnvNS3 Protease. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Viral wnvNS3 Protease Monoclonal Antibody (Catalog # MAB29071) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for full length wnvNS3 Protease at approximately 32 kDa and C-terminal NS3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Viral wnvNS3 Protease Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Viral wnvNS3 Protease (Catalog # 2907-SE), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Viral wnvNS3 Protease (Catalog # 2907-SE), see our available Western blot detection antibodies
Western Blot
2 µg/mL
Sample: Recombinant Viral wnvNS3 Protease (Catalog # 2907-SE)
Sample: Recombinant Viral wnvNS3 Protease (Catalog # 2907-SE)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile Tris-buffered saline, pH 7.3. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: wnvNS3 Protease
Infection of mosquito-borne West Nile Virus can cause severe neurological disease and can be epidemic. Two non-structural proteins, NS3 and NS2b, play an essential role in viral replication and are therefore a potential target for treatment and prevention of West Nile Virus disease. NS3 consists of a trypsin-like serine protease with a catalytic triad (His51, Asp75, Ser135) and a putative helicase. Requiring NS2b as the cofactor, NS3 protease processes viral polyprotein precursor (1, 2). The purified recombinant protein used as an immunogen consists of three forms: the full-length fusion protein, the N-terminal NS2b, and the C-terminal NS3 with a G4SG4 linker. NS3 protease has a relatively narrow substrate specificity that prefers Arg in P1 and Lys in P2.
References
- Nall, T.A. et al. (2004) J. Biol. Chem. 279:48535.
- Chappell, K.J. et al. (2005) J. Biol. Chem. 274:2896.
Long Name
West Nile Virus NS3 Protease
Entrez Gene IDs
912267 (Viral)
Gene Symbol
POLY
UniProt
Additional wnvNS3 Protease Products
Product Documents for Viral wnvNS3 Protease Antibody
Product Specific Notices for Viral wnvNS3 Protease Antibody
For research use only
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