Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Virilizer Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1760 and 1810 of human Virilizer (KIAA1429) using the numbering given in entry NP_056311.2 (GeneID 25962).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Virilizer Antibody

Western Blot: Virilizer Antibody [NBP1-21364]

Western Blot: Virilizer Antibody [NBP1-21364]

Western Blot: Virilizer Antibody [NBP1-21364] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Virilizer antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: Virilizer Antibody [NBP1-21364]

Immunoprecipitation: Virilizer Antibody [NBP1-21364]

Immunoprecipitation: Virilizer Antibody [NBP1-21364] - Detection of human Virilizer by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Virilizer antibody NBP1-21364 used for IP at 6 ug per reaction. Virilizer was also immunoprecipitated by another rabbit anti-Virilizer antibody. For blotting immunoprecipitated Virilizer, NBP1-21364 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Virilizer Antibody

Application
Recommended Usage

Immunoprecipitation

5-10 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Virilizer

The virilizer (vir) gene was discovered as a factor required for dosage compensation and sex determination in female flies. In drosophila it has been determined to function as a positive regulator of female-specific mRNA splicing. Database searches using the deduced amino acid sequence of the drosophila VIR retrieved a human protein BAA92667 (GenBank) which corresponds to the gene designated as KIAA1429. The human protein is 28% identical and about 40% similar to drosophila VIR and contains the putative transmembrane domain of drosophila VIR.

Alternate Names

DKFZp434I116, DKFZp781B2117, fSAP121, functional spliceosome-associated protein 121, KIAA1429, MGC138493, MGC141940, protein virilizer homolog

Entrez Gene IDs

25962 (Human)

Gene Symbol

VIRMA

UniProt

NP_056311.2 (Human)

Additional Virilizer Products

Product Documents for Virilizer Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Virilizer Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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