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Virilizer Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93159

Novus Biologicals, part of Bio-Techne
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NBP2-93159-0.1ml
NBP2-93159-0.02ml

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1400-1700 of human KIAA1429 (NP_056311.2). FMRQILNSDTIGCCGDDNGLMEVEGAHTSRTMSINAAELKQLLQSKEESPENLFLELEKLVLEHSKDDDNLDSLLDSVVGLKQMLESSGDPLPLSDQDVEPVLSAPESLQNLFNNRTAYVLADVMDDQLKSMWFTPFQAEEIDTDLDLVKVDLIELSEKCCSDFDLHSELERSFLSEPSSPGRTKTTKGFKLGKHKHETFITSSGKSEYIEPAKRAHVVPPPRGRGRGGFGQGIRPHDIFRQRKQNTSRPPSMHVDDFVAAESKEVVPQDGIPPPKRPLKVSQKISSRGGFSGNRGGRGAF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

202 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Virilizer Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: Virilizer Antibody - Azide and BSA Free [NBP2-93159]

Immunohistochemistry-Paraffin: Virilizer Antibody - Azide and BSA Free [NBP2-93159]

Immunohistochemistry-Paraffin: Virilizer Antibody [NBP2-93159] - Mouse brain using KIAA1429 antibody at dilution of 1:100 (40x lens).
Virilizer Antibody - Azide and BSA Free

Western Blot: Virilizer Antibody [NBP2-93159] -

Western Blot: Virilizer Antibody [NBP2-93159] - Analysis of extracts of Mouse lung, using KIAA1429 antibody at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
Virilizer Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: Virilizer Antibody [NBP2-93159] -

Immunohistochemistry-Paraffin: Virilizer Antibody [NBP2-93159] - Mouse kidney using KIAA1429 Rabbit pAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Applications for Virilizer Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:50 - 1:200

Western Blot

1:1000 - 1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Virilizer

The virilizer (vir) gene was discovered as a factor required for dosage compensation and sex determination in female flies. In drosophila it has been determined to function as a positive regulator of female-specific mRNA splicing. Database searches using the deduced amino acid sequence of the drosophila VIR retrieved a human protein BAA92667 (GenBank) which corresponds to the gene designated as KIAA1429. The human protein is 28% identical and about 40% similar to drosophila VIR and contains the putative transmembrane domain of drosophila VIR.

Alternate Names

DKFZp434I116, DKFZp781B2117, fSAP121, functional spliceosome-associated protein 121, KIAA1429, MGC138493, MGC141940, protein virilizer homolog

Gene Symbol

VIRMA

Additional Virilizer Products

Product Documents for Virilizer Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Virilizer Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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