visinin-like 1 Antibody (2D11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-25166

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2D11

Concentration

1 mg/ml

View all VSNL1 Antibodies »

Product Specifications

Immunogen

Full length human visinin-like 1 [UniProt# P62760]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for visinin-like 1 Antibody (2D11)

Western Blot: visinin-like 1 Antibody (2D11) [NBP2-25166]

Western Blot: visinin-like 1 Antibody (2D11) [NBP2-25166]

Western Blot: visinin-like 1 Antibody (2D11) [NBP2-25166] - Western blot analysis of different tissue lysates using mouse mAb to Visinin-like Protein 1 (VLP1), NBP2-25166, dilution 1:1,000 in green: [1] protein standard (red), [2] rat brain [3] rat cerebellum, and [4] mouse brain. The band at around 20kDa mark corresponds to VLP1 protein.
Immunohistochemistry Free-Floating: visinin-like 1 Antibody (2D11) [NBP2-25166]

Immunohistochemistry Free-Floating: visinin-like 1 Antibody (2D11) [NBP2-25166]

Immunohistochemistry Free-Floating: visinin-like 1 Antibody (2D11) [NBP2-25166] - Immunofluorescent analysis of rat cerebellum section stained with mouse mAb to VLP1, NBP2-25166, dilution 1:500, in red and costained with rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with the above antibodies. The VLP1 antibody reveals protein expressed in granule cell membranes and in synapses in the white matter and molecular layers of the cerebellum. The GFAP antibody stains the processes of Bergmann glia and astroglia.
Immunohistochemistry: visinin-like 1 Antibody (2D11) [NBP2-25166]

Immunohistochemistry: visinin-like 1 Antibody (2D11) [NBP2-25166]

Immunohistochemistry: visinin-like 1 Antibody (2D11) [NBP2-25166] - Confocal image of adult rat cerebellar cortex stained with NBP2-25166 (green), chicken polyclonal antibody to MAP2 NB300-213 (red) and DNA (blue). NBP2-25166 reveals synapses in the molecular layer (ML) strongly. Synaptic regions are also seen in the granule cell layer (GC). The perikarya of Purkinje cells (PC) are revealed with MAP2 antibody. Little staining is seen in the white matter (WM).

Applications for visinin-like 1 Antibody (2D11)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1:1000

Immunohistochemistry

1:500-1:1000

Western Blot

1:500-1:1000
Application Notes
This visinin-like 1 (2D11) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry, and Western Blot, where a band can be seen at ~22 kDa.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VSNL1

Visinin was originally isolated biochemically from chicken retina as a major protein of about 24 kDa on SDS-PAGE. Following cloning and sequencing of visinin, several visinin-like proteins were discovered by homology screening. One of these, visinin-like 1 is a small calcium binding protein which is very abundant in the nervous system and is found only in neurons, though different neurons have different levels of expression. It is particularly concentrated in cerebellar Purkinje cells, and tends to be most abundant in perikarya and dendrites. The protein was discovered independently by several groups and is therefore also sometimes known as hippocalcin-like protein 3, HLP3, HPCAL3, HUVISL1, VLP-1, VILIP and VILIP-1. The protein belongs to the large superfamily of calmodulin and paravalbumin type proteins which function by binding calcium ions. Calcium binding alters the conformation of these proteins and allow them to interact with other binding partners, the properties of which they may alter. Visinin-like 1 has four "EF hand" domains, which are negatively charged helix-turn-helix peptides that are responsible for calcium binding. Visinin-like 1 is 191 amino acids in size and has a molecular weight on SDS-PAGE of ~22kDa. The protein has recently been suggested to be a useful biomarker of Alzheimer's disease and traumatic brain injury.

Long Name

Visinin Like 1

Alternate Names

HPCAL3, HUVISL1, VILIP, VISL1

Entrez Gene IDs

7447 (Human)

Gene Symbol

VSNL1

OMIM

600817 (Human)

UniProt

P62760 (Human)

Additional VSNL1 Products

Product Documents for visinin-like 1 Antibody (2D11)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for visinin-like 1 Antibody (2D11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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