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visinin-like 1 Antibody (2F1-E3)

Novus Biologicals, part of Bio-Techne | Catalog # H00007447-M01

Novus Biologicals, part of Bio-Techne
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H00007447-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F1-E3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

VSNL1 (AAH22012, 1 a.a. ~ 191 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGKQNSKLAPEVMEDLVKSTEFNEHELKQWYKGFLKDCPSGRLNLEEFQQLYVKFFPYGDASKFAQHAFRTFDKNGDGTIDFREFICALSITSRGSFEQKLNWAFNMYDLDGDGKITRVEMLEIIEAIYKMVGTVIMMKMNEDGLTPEQRVDKIFSKMDKNKDDQITLDEFKEAAKSDPSIVLLLQCDIQK

Specificity

VSNL1 - visinin-like 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for visinin-like 1 Antibody (2F1-E3)

Western Blot: visinin-like 1 Antibody (2F1-E3) [H00007447-M01]

Western Blot: visinin-like 1 Antibody (2F1-E3) [H00007447-M01]

Western Blot: visinin-like 1 Antibody (2F1-E3) [H00007447-M01] - Analysis of VSNL1 expression in transfected 293T cell line by VSNL1 monoclonal antibody (M01), clone 2F1-E3.Lane 1: VSNL1 transfected lysate(22.1 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: visinin-like 1 Antibody (2F1-E3) [H00007447-M01] - Detection limit for recombinant GST tagged VSNL1 is approximately 0.3ng/ml as a capture antibody.

Applications for visinin-like 1 Antibody (2F1-E3)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: VSNL1

This gene is a member of the visinin/recoverin subfamily of neuronal calcium sensor proteins. The encoded protein is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium-dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.

Long Name

Visinin Like 1

Alternate Names

HPCAL3, HUVISL1, VILIP, VISL1

Entrez Gene IDs

7447 (Human)

Gene Symbol

VSNL1

OMIM

600817 (Human)

Additional VSNL1 Products

Product Documents for visinin-like 1 Antibody (2F1-E3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for visinin-like 1 Antibody (2F1-E3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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