VISTA/B7-H5/PD-1H Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88967

Novus Biologicals, part of Bio-Techne
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NBP1-88967
NBP1-88967-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VISTA/B7-H5/PD-1H Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LTFQDLHLHHGGHQAANTSHDLAQRHGLESASDHHGNFSITMRNLTLLDSGLYCCLVVEIRHHHSEHRVHGAMELQVQTGKDAPSNCVVYPSSS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VISTA/B7-H5/PD-1H Antibody - BSA Free

Western Blot: VISTA/B7-H5/PD-1H Antibody [NBP1-88967]

Western Blot: VISTA/B7-H5/PD-1H Antibody [NBP1-88967]

Western Blot: VISTA/B7-H5/PD-1H Antibody [NBP1-88967] - Analysis in human cell lines A-431 and HEK293 using anti-C10orf54 antibody. Corresponding C10orf54 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Immunohistochemistry-Paraffin: VISTA/B7-H5/PD-1H Antibody [NBP1-88967]

Immunohistochemistry-Paraffin: VISTA/B7-H5/PD-1H Antibody [NBP1-88967]

Immunohistochemistry-Paraffin: VISTA/B7-H5/PD-1H Antibody [NBP1-88967] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.
Immunohistochemistry-Paraffin: VISTA/B7-H5/PD-1H Antibody [NBP1-88967]

Immunohistochemistry-Paraffin: VISTA/B7-H5/PD-1H Antibody [NBP1-88967]

Immunohistochemistry-Paraffin: VISTA/B7-H5/PD-1H Antibody [NBP1-88967] - Staining of human Fallopian tube shows strong cytoplasmic positivity in leukocytes.
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Applications for VISTA/B7-H5/PD-1H Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 -1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VISTA/B7-H5/PD-1H

VISTA, also known as B7-H5, PD-1H, platelet receptor Gi24, Dies1, and SISP1, is a transmembrane glycoprotein that promotes both MT1-MMP expression and the MT1-MMP mediated activation of MMP-2. VISTA supports the differentiation of embryonic stem cells (ESC) and enhances BMP-4 induced signaling in ESC but is also downregulated following BMP-4 exposure. It binds to BMP-4 directly and also associates with the type I BMP receptor Activin RIB/ALK-4. VISTA is expressed on the surface of naïve CD4+ T cells, regulatory T cells, and adipocytes. It is upregulated in vivo on activated monocytes and dendritic cells. VISTA inhibits CD4+ and CD8+ T cell proliferation and their production of IL-2 and IFN-gamma. Its expression on tumor cells attenuates the anti-tumor immune response and enables more rapid tumor progression. In contrast, VISTA limits disease progression in the autoimmune disease model EAE.

Alternate Names

4632428N05Rik, B7-H5, C10orf54, Dies1, Gi24, PD-1H, PD1H, SISP1, VSIR

Gene Symbol

VSIR

Additional VISTA/B7-H5/PD-1H Products

Product Documents for VISTA/B7-H5/PD-1H Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VISTA/B7-H5/PD-1H Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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