Vitamin D BP Antibody (VDBP/4481)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13923

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # VDBP/4481

Concentration

0.2 mg/ml

View all Vitamin D BP Antibodies »

Product Summary for Vitamin D BP Antibody (VDBP/4481)

Immunogen

Recombinant fragment (around aa 35-175) of human Vitamin D BP protein (exact sequence is proprietary) (Uniprot: P02774)

Localization

Secreted

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14005)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Vitamin D BP Antibody (VDBP/4481)

Immunohistochemistry-Paraffin: Vitamin D BP Antibody (VDBP/4481) [NBP3-13923]

Immunohistochemistry-Paraffin: Vitamin D BP Antibody (VDBP/4481) [NBP3-13923]

Immunohistochemistry-Paraffin: Vitamin D BP Antibody (VDBP/4481) [NBP3-13923] - IHC analysis of formalin-fixed, paraffin-embedded human pancreas. Staining using Vitamin D BP antibody (VDBP/4481) at 2ug/ml in PBS for 30min RT.
Immunohistochemistry-Paraffin: Vitamin D BP Antibody (VDBP/4481) [NBP3-13923]

Immunohistochemistry-Paraffin: Vitamin D BP Antibody (VDBP/4481) [NBP3-13923]

Immunohistochemistry-Paraffin: Vitamin D BP Antibody (VDBP/4481) [NBP3-13923] - IHC analysis of formalin-fixed, paraffin-embedded human renal oncocytoma. Staining using Vitamin D BP antibody (VDBP/4481) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Protein Array: Vitamin D BP Antibody (VDBP/4481) [NBP3-13923]

Protein Array: Vitamin D BP Antibody (VDBP/4481) [NBP3-13923]

Protein Array: Vitamin D BP Antibody (VDBP/4481) [NBP3-13923] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Vitamin D BP Antibody (VDBP/4481).

Applications for Vitamin D BP Antibody (VDBP/4481)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Vitamin D BP

Vitamin D Binding Protein is a secreted multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and urine, and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane bound immunoglobulin on the surface of B lymphocytes and with IgG fc receptor on the membranes of T lymphocytes.

Long Name

Vitamin D Binding Protein

Alternate Names

DBP, DBP-MAF, Gc, Gc-MAF, VDBG, VDBP

Gene Symbol

GC

UniProt

P02774

Additional Vitamin D BP Products

Product Documents for Vitamin D BP Antibody (VDBP/4481)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Vitamin D BP Antibody (VDBP/4481)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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