Vitronectin Antibody (2V2V8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16498

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16498-100ul
NBP3-16498-20ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2V2V8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Vitronectin Antibodies »

Product Summary for Vitronectin Antibody (2V2V8)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 379-478 of human Vitronectin (P04004). YRSQRGHSRGRNQNSRRPSRATWLSLFSSEESNLGANNYDDYRMDWLVPATCEPIQSVFFFSGDKYYRVNLRTRRVDTVDPPYPRSIAQYWLGCPAPGHL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Vitronectin Antibody (2V2V8)

Western Blot: Vitronectin Antibody (2V2V8) [NBP3-16498]

Western Blot: Vitronectin Antibody (2V2V8) [NBP3-16498]

Western Blot: Vitronectin Antibody (2V2V8) [NBP3-16498] - Western blot analysis of extracts of HepG2 cells, using Vitronectin Rabbit mAb (NBP3-16498) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Applications for Vitronectin Antibody (2V2V8)

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Vitronectin

Vitronectin also called serum spreading factor or complement S-protein, is a member of the pexin family (1). Found in serum and in tissues, Vitronectin exist in two forms; as a single chain (75kDa) and as a clipped form composed of two chains (65kDa and 10kDa). Vitronectin promotes cell adhesion, spreading and migration through specific interactions with cellular integrins, and inhibits cytolysis by the complement C5b-9 complex (2-4). Vitronectin also interacts with several critical proteins that regulate thrombosis and fibrinolysis. Vitronectin is phosphorylated by PKA and CKII (5).

Alternate Names

Complement S-protein, Serum Spreading Factor, Somatomedin B, VTN

Gene Symbol

VTN

Additional Vitronectin Products

Product Documents for Vitronectin Antibody (2V2V8)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Vitronectin Antibody (2V2V8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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