Von Willebrand Factor Antibody (3E2D10) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34535

Novus Biologicals, part of Bio-Techne
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NBP2-34535-0.1mg
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3E2D10

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Von Willebrand Factor Antibodies »

Product Specifications

Immunogen

Recombinant human Von Willebrand Factor fragment spanning around aa 845-949 (exact sequence is proprietary) (Uniprot: P04275 )

Localization

Cytoplasmic

Specificity

von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.

Marker

Endothelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

250 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-33002).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Von Willebrand Factor Antibody (3E2D10) - Azide and BSA Free

Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody (3E2D10) - Azide and BSA Free [NBP2-34535]

Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody (3E2D10) - Azide and BSA Free [NBP2-34535]

Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody (3E2D10) - Azide and BSA Free [NBP2-34535] - Formalin-fixed, paraffin-embedded human brain stained using Von Willebrand Factor Antibody (3E2D10) followed by goat anti-mouse IgG-CF488 (green). DAPI used to label nuclei.
Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody (3E2D10) - Azide and BSA Free [NBP2-34535]

Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody (3E2D10) - Azide and BSA Free [NBP2-34535]

Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody (3E2D10) - Azide and BSA Free [NBP2-34535] - Formalin-fixed, paraffin-embedded human tonsil stained with vWF MAb (3E2D10)
Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody (3E2D10) - Azide and BSA Free [NBP2-34535]

Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody (3E2D10) - Azide and BSA Free [NBP2-34535]

Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody (3E2D10) - Azide and BSA Free [NBP2-34535] - Formalin-fixed, paraffin-embedded human Pancreas stained with Von Willebrand Factor Antibody (3E2D10).

Applications for Von Willebrand Factor Antibody (3E2D10) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-1ug/ml

Immunohistochemistry-Paraffin

0.5-1.0ug/ml

Immunoprecipitation

0.5-1ug/500ug protein lysate

Western Blot

0.5-1.0ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Von Willebrand Factor

The glycoprotein encoded by this gene functions as both an antihemophilic factor carrier and a platelet-vessel wall mediator in the blood coagulation system. It is crucial to the hemostasis process. Mutations in this gene or deficiencies in this protein result in von Willebrand's disease. An unprocessed pseudogene has been found on chromosome 22.

Alternate Names

coagulation factor VIII VWF, F8, F8VWF, von Willebrand factor, VWD, vWF

Gene Symbol

VWF

Additional Von Willebrand Factor Products

Product Documents for Von Willebrand Factor Antibody (3E2D10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Von Willebrand Factor Antibody (3E2D10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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