Von Willebrand Factor Antibody (SPM577)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33004
![Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody (SPM577) [NBP2-33004]](https://resources.bio-techne.com/images/products/Von-Willebrand-Factor-Antibody-SPM577-Immunohistochemistry-Paraffin-NBP2-33004-img0001.jpg "Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody (SPM577) [NBP2-33004]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM577
Concentration
0.2 mg/ml
Product Summary for Von Willebrand Factor Antibody (SPM577)
Immunogen
Recombinant human Von Willebrand Factor fragment (Uniprot: P04275 )
Localization
Cytoplasmic
Specificity
von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.
Marker
Endothelial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
250 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34810)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Von Willebrand Factor Antibody (SPM577)
Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody (SPM577) [NBP2-33004]
Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody (SPM577) [NBP2-33004] - Formalin-paraffin tonsil stained with vWF MAb (SPM577)Applications for Von Willebrand Factor Antibody (SPM577)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Von Willebrand Factor
Alternate Names
coagulation factor VIII VWF, F8, F8VWF, von Willebrand factor, VWD, vWF
Gene Symbol
VWF
UniProt
Additional Von Willebrand Factor Products
Product Documents for Von Willebrand Factor Antibody (SPM577)
Product Specific Notices for Von Willebrand Factor Antibody (SPM577)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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