Von Willebrand Factor Antibody (VWF/1767)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53272
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # VWF/1767
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa1815-1939) of human Von Willebrand Factor protein (exact sequence is proprietary) (Uniprot: P04275)
Localization
Cytoplasmic
Specificity
von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposis sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.
Marker
Endothelial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
250 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54497)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Von Willebrand Factor Antibody (VWF/1767)
Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody (VWF/1767) [NBP2-53272]
Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody (VWF/1767) [NBP2-53272] - Formalin-fixed, paraffin-embedded human Spleen stained with vWF Monoclonal Antibody (VWF/1767)Protein Array: Von Willebrand Factor Antibody (VWF/1767) [NBP2-53272]
Protein Array: Von Willebrand Factor Antibody (VWF/1767) [NBP2-53272] - Analysis of Protein Array containing >19,000 full-length human proteins using Von Willebrand Factor Antibody (VWF/1767) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.Applications for Von Willebrand Factor Antibody (VWF/1767)
Application
Recommended Usage
ELISA
2-4 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Von Willebrand Factor
Alternate Names
coagulation factor VIII VWF, F8, F8VWF, von Willebrand factor, VWD, vWF
Gene Symbol
VWF
UniProt
Additional Von Willebrand Factor Products
Product Documents for Von Willebrand Factor Antibody (VWF/1767)
Product Specific Notices for Von Willebrand Factor Antibody (VWF/1767)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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