Von Willebrand Factor Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-586

Novus Biologicals, part of Bio-Techne
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NB600-586-0.025ml
NB600-586-0.1ml
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Canine, Feline

Cited:

Human, Mouse, Canine, Feline

Applications

Validated:

Dual RNAscope ISH-IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Von Willebrand Factor Antibodies »

Product Specifications

Immunogen

Purified antisera of the Von Willebrand Factor (factor VIII R:ag) related antigen in plasma.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31738896). Feline reactivity reported in scientific literature (PMID:31616579).

Localization

Secreted. Localized to storage granules.

Specificity

The antibody specifically reacts with the cytoplasm of human endothelial cells from normal and neoplastic blood and from lymphatic vessels. It also reacts with human endocardium, platelets and megakaryocytes. It may be used for immunohistological studies of the vascular structures in frozen tissue preparations and enzymatically pre-digested, formalin, methacarn, Bouin's or ethanol-fixed and paraffin-embedded tissue sections.

Marker

Endothelial Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Von Willebrand Factor Antibody

Dual RNAscope ISH-IHC: Von Willebrand Factor Antibody [NB600-586] - Formalin-fixed paraffin-embedded tissue sections of human metastatic tonsil were probed for vWF mRNA (ACD RNAScope probe, catalog # 5860461; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal (Novus catalog # NB600-586) at 1:500 dilution for 1 hour at room temperature followed by incubation with the anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).
Immunohistochemistry: Von Willebrand Factor Antibody [NB600-586]

Immunohistochemistry: Von Willebrand Factor Antibody [NB600-586]

Immunohistochemistry: Von Willebrand Factor Antibody [NB600-586] - Formalin fixed paraffin embedded human tonsil stained with Factor VIII antibody.
Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody [NB600-586]

Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody [NB600-586]

Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody [NB600-586] - Staining of formalin-fixed, paraffin-embedded Human Kidney sections with 1:2,000 Rabbit Anti-von Willebrand Factor.
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Applications for Von Willebrand Factor Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000
Application Notes
IHC-P: We recommend proteolycic antigen retrieval. After deparaffinization treat with 0.1% Pronase in PBS for 5-10 minutes at 37 degrees C. Alternatively, digest with 0.1% Trypsin in PBS for 25 minutes at 37 degrees C. Gently rinse in PBS. This Von Willebrand Factor Antibody is validated for IHC-Fr from a verified customer review.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 4.3 using NB600-586 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

10 mM PBS (pH 7.4)

Preservative

0.9% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Von Willebrand Factor

Human Von Willebrand Factor(factor VIII R:Ag) is a 270 kDa multimeric plasma gylcoprotein. It mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation factor VIII. The von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. The factor is synthesized by endothelial cells and is also present in platelets and megakaryocytes.

Alternate Names

coagulation factor VIII VWF, F8, F8VWF, von Willebrand factor, VWD, vWF

Entrez Gene IDs

7450 (Human)

Gene Symbol

VWF

UniProt

P04275

Additional Von Willebrand Factor Products

Product Documents for Von Willebrand Factor Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Von Willebrand Factor Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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