Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all VPS11 Antibodies »

Product Summary for VPS11 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 550 and 600 of human Vacuolar Protein Sorting 11 using the numbering given in entry NP_068375.3 (GeneID 55823).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VPS11 Antibody

Western Blot: VPS11 Antibody [NBP2-04092]

Western Blot: VPS11 Antibody [NBP2-04092]

Western Blot: VPS11 Antibody [NBP2-04092] - Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-VPS11 antibody NBP2-04092 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. VPS11 was also immunoprecipitated by rabbit anti-VPS11 antibody NBP2-04093, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Applications for VPS11 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 mcg/mg

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: VPS11

VPS11 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

Alternate Names

hVPS11, PEP5, RING finger protein 108, RNF108END1, vacuolar protein sorting 11 (yeast homolog), vacuolar protein sorting 11 homolog (S. cerevisiae), vacuolar protein sorting protein 11, vacuolar protein sorting-associated protein 11 homolog

Gene Symbol

VPS11

UniProt

NP_068375.3 (Human)

Additional VPS11 Products

Product Documents for VPS11 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VPS11 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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