VPS25 Antibody (2E5-2B9)

Novus Biologicals, part of Bio-Techne | Catalog # H00084313-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2E5-2B9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VPS25 Antibodies »

Product Specifications

Immunogen

MGC10540 (AAH06282, 1 a.a. ~ 176 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAMSFEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEAQESPLFNNVKLQRKLPVESIQIVLEELRKKGNLEWLDKSKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSVFTLYELTNGEDTEDEEFHGLDEATLLRALQALQQEHKAEIITVSDGRGVKFF

Reactivity Notes

Human. Other species not tested.

Specificity

VPS25 - hypothetical protein MGC10540

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for VPS25 Antibody (2E5-2B9)

Western Blot: VPS25 Antibody (2E5-2B9) [H00084313-M01]

Western Blot: VPS25 Antibody (2E5-2B9) [H00084313-M01]

Western Blot: VPS25 Antibody (2E5-2B9) [H00084313-M01] - MGC10540 monoclonal antibody (M01), clone 2E5-2B9 Analysis of MGC10540 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: VPS25 Antibody (2E5-2B9) [H00084313-M01]

Immunocytochemistry/ Immunofluorescence: VPS25 Antibody (2E5-2B9) [H00084313-M01]

Immunocytochemistry/Immunofluorescence: VPS25 Antibody (2E5-2B9) [H00084313-M01] - Analysis of monoclonal antibody to VPS25 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: VPS25 Antibody (2E5-2B9) [H00084313-M01] - Detection limit for recombinant GST tagged VPS25 is approximately 0.03ng/ml as a capture antibody.

Applications for VPS25 Antibody (2E5-2B9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: VPS25

VPS25, VPS36 (MIM 610903), and SNF8 (MIM 610904) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. VPS25, VPS36, and SNF8 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).[supplied by OMIM]

Alternate Names

Dermal papilla-derived protein 9, DERP9, DERP9hVps25, EAP20, EAP20FAP20, ELL-associated protein of 20 kDa, ESCRT-II complex subunit VPS25, FAP20, MGC10540, vacuolar protein sorting 25 (yeast), vacuolar protein sorting 25 homolog (S. cerevisiae), vacuolar protein-sorting-associated protein 25

Entrez Gene IDs

84313 (Human)

Gene Symbol

VPS25

UniProt

AAH06282 (Human)

Additional VPS25 Products

Product Documents for VPS25 Antibody (2E5-2B9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VPS25 Antibody (2E5-2B9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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