VPS25 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2468

Novus Biologicals, part of Bio-Techne
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NB100-2468
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Key Product Details

Species Reactivity

Validated:

Rat

Predicted:

Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all VPS25 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QPNVDTRQKQ corresponding to internal region according to NP_115729.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for VPS25 Antibody

Western Blot: VPS25 Antibody [NB100-2468]

Western Blot: VPS25 Antibody [NB100-2468]

Western Blot: VPS25 Antibody [NB100-2468] - Staining of Rat Kidney lysate (35 ug protein in RIPA buffer) with antibody at 0.5 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: VPS25 Antibody [NB100-2468]

Immunohistochemistry-Paraffin: VPS25 Antibody [NB100-2468]

Immunohistochemistry-Paraffin: VPS25 Antibody [NB100-2468] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Immunohistochemistry-Paraffin: VPS25 Antibody [NB100-2468]

Immunohistochemistry-Paraffin: VPS25 Antibody [NB100-2468]

Immunohistochemistry-Paraffin: VPS25 Antibody [NB100-2468] - (6ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for VPS25 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

6-8 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1ug/mL
Application Notes
Western blot: Approx. 22kDa band observed in Rat Kidney lysates (calculated MW of 20.7kDa according to human NP_115729.1).
IHC: In paraffin embedded human kidney, shows granular cytoplasmic staining of endothelial cells. Data obtained from the previous batch (different goat).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VPS25

Gene ID(s): 84313 (human)

Alternate Names

Dermal papilla-derived protein 9, DERP9, DERP9hVps25, EAP20, EAP20FAP20, ELL-associated protein of 20 kDa, ESCRT-II complex subunit VPS25, FAP20, MGC10540, vacuolar protein sorting 25 (yeast), vacuolar protein sorting 25 homolog (S. cerevisiae), vacuolar protein-sorting-associated protein 25

Entrez Gene IDs

84313 (Human)

Gene Symbol

VPS25

UniProt

NP_115729.1 (Human)

Additional VPS25 Products

Product Documents for VPS25 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VPS25 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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