VPS29 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20871

Novus Biologicals, part of Bio-Techne
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NBP2-20871
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Chicken (96%), Mouse (98%), Rat (97%), Xenopus (95%), Zebrafish (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VPS29 Antibodies »

Product Summary for VPS29 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human VPS29. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (95%), Drosophila (80%).

Localization

Cytoplasm; Membrane; Peripheral membrane protein; Endosome membrane; Peripheral membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VPS29 Antibody

Western Blot: VPS29 Antibody [NBP2-20871]

Western Blot: VPS29 Antibody [NBP2-20871]

Western Blot: VPS29 Antibody [NBP2-20871] - Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE gel, diluted at 1:2000.
Immunohistochemistry-Paraffin: VPS29 Antibody [NBP2-20871]

Immunohistochemistry-Paraffin: VPS29 Antibody [NBP2-20871]

Immunohistochemistry-Paraffin: VPS29 Antibody [NBP2-20871] - Human breast cancer, using VPS29 antibody at 1:250 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15 min.

Applications for VPS29 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: VPS29

This gene belongs to a group of vacuolar protein sorting (VPS) genes that, when functionally impaired, disrupt the efficient delivery of vacuolar hydrolases. The protein encoded by this gene is a component of a large multimeric complex, termed the retromer complex, which is involved in retrograde transport of proteins from endosomes to the trans-Golgi network. This VPS protein may be involved in the formation of the inner shell of the retromer coat for retrograde vesicles leaving the prevacuolar compartment. Alternative splice variants encoding different isoforms, and usage of multiple polyadenylation sites have been found for this gene. [provided by RefSeq]

Alternate Names

DC15, DC7, DKFZp564F0223, EC 3.1.3.3, FLJ20492, hVPS29, PEP11, PEP11 homolog, retromer protein, vacuolar protein sorting 29 (yeast homolog), vacuolar protein sorting 29 (yeast), vacuolar protein sorting 29 homolog (S. cerevisiae), vacuolar protein sorting-associated protein 29, vacuolar sorting protein VPS29/PEP11, Vesicle protein sorting 29, x 007 protein

Gene Symbol

VPS29

UniProt

Q9UBQ0 (Human)

Additional VPS29 Products

Product Documents for VPS29 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VPS29 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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