VPS34 Antibody (9U5O3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15441

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15441-100ul
NBP3-15441-20ul
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9U5O3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all VPS34/PIK3C3 Antibodies »

Product Summary for VPS34 Antibody (9U5O3)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 788-887 of human VPS34 (Q8NEB9). GGTQSEQYQEFRKQCYTAFLHLRRYSNLILNLFSLMVDANIPDIALEPDKTVKKVQDKFRLDLSDEEAVHYMQSLIDESVHALFAAVVEQIHKFAQYWRK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VPS34 Antibody (9U5O3)

Western Blot: VPS34 Antibody (9U5O3) [NBP3-15441]

Western Blot: VPS34 Antibody (9U5O3) [NBP3-15441]

Western Blot: VPS34 Antibody (9U5O3) [NBP3-15441] - Western blot analysis of extracts of various cell lines, using VPS34 Rabbit mAb (NBP3-15441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: VPS34 Antibody (9U5O3) [NBP3-15441]

Immunocytochemistry/ Immunofluorescence: VPS34 Antibody (9U5O3) [NBP3-15441]

Immunocytochemistry/Immunofluorescence: VPS34 Antibody (9U5O3) [NBP3-15441] - Immunofluorescence analysis of NIH-3T3 cells using VPS34 Rabbit mAb (NBP3-15441) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation: VPS34 Antibody (9U5O3) [NBP3-15441]

Immunoprecipitation: VPS34 Antibody (9U5O3) [NBP3-15441]

Immunoprecipitation: VPS34 Antibody (9U5O3) [NBP3-15441] - Immunoprecipitation analysis of 600ug extracts of Rat brain cells using 3ug VPS34 antibody (NBP3-15441). Western blot was performed from the immunoprecipitate using VPS34 antibody (NBP3-15441) at a dilition of 1:500.

Applications for VPS34 Antibody (9U5O3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

1:100 - 1:500

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VPS34/PIK3C3

VPS34, also known as phosphoinositide-3-kinase p100 subunit or PIK3C3 (phosphoinositide-3-kinase, class III), is a member of the PI3/PI4-kinase family. It is ubiquitously expressed with predominant expression in skeletal muscle and is believed to participate in endosome to lysosome transport, multivesicular body formation, autophagy and retrograde endosome to Golgi transport. VPS34 is the catalytic subunit of class III PI3Ks and forms a heterodimer with p150, a regulatory subunit of class 3 PI3Ks. It exclusively phosphorylates phosphatidylinositol to produce PtdIns3P. The activity of VPS34 is enhanced by manganese and can be regulated by nutrients, suggesting an important role of VPS34 in the regulation of mTOR protein synthesis. VPS34 also forms a complex with Beclin1 that promotes autophagy and tumor supression.

Alternate Names

PI 3-Kinase C3, PI3K type 3, PIK3C3

Gene Symbol

PIK3C3

Additional VPS34/PIK3C3 Products

Product Documents for VPS34 Antibody (9U5O3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for VPS34 Antibody (9U5O3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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