VPS35 Antibody (2D3)

Novus Biologicals, part of Bio-Techne | Catalog # H00055737-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2D3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VPS35 Antibodies »

Product Specifications

Immunogen

VPS35 (NP_060676, 697 a.a. ~ 796 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25190809). Other species not tested.

Specificity

VPS35 (2D3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for VPS35 Antibody (2D3)

Western Blot: VPS35 Antibody (2D3) [H00055737-M02]

Western Blot: VPS35 Antibody (2D3) [H00055737-M02]

Western Blot: VPS35 Antibody (2D3) [H00055737-M02] - VPS35 monoclonal antibody (M02), clone 2D3. Analysis of VPS35 expression in human colon.
Western Blot: VPS35 Antibody (2D3) [H00055737-M02]

Western Blot: VPS35 Antibody (2D3) [H00055737-M02]

Western Blot: VPS35 Antibody (2D3) [H00055737-M02] - VPS35 monoclonal antibody (M02), clone 2D3. Analysis of VPS35 expression in HeLa.
ELISA: VPS35 Antibody (2D3) [H00055737-M02]

ELISA: VPS35 Antibody (2D3) [H00055737-M02]

ELISA: VPS35 Antibody (2D3) [H00055737-M02] - Detection limit for recombinant GST tagged VPS35 is 0.03 ng/ml as a capture antibody.

Applications for VPS35 Antibody (2D3)

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 28146471).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: VPS35

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.

Alternate Names

DKFZp434P1672, FLJ10752, FLJ13588, FLJ20388, hVPS35, Maternal-embryonic 3, MEM3DKFZp434E1211, vacuolar protein sorting 35 (yeast homolog), vacuolar protein sorting 35 (yeast), vacuolar protein sorting 35 homolog (S. cerevisiae), vacuolar protein sorting-associated protein 35, Vesicle protein sorting 35

Entrez Gene IDs

55737 (Human)

Gene Symbol

VPS35

OMIM

606931 (Human)

UniProt

NP_060676 (Human)

Additional VPS35 Products

Product Documents for VPS35 Antibody (2D3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VPS35 Antibody (2D3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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