VPS35 Antibody (2D3)
Novus Biologicals, part of Bio-Techne | Catalog # H00055737-M02
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2D3
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
VPS35 (NP_060676, 697 a.a. ~ 796 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 25190809). Other species not tested.
Specificity
VPS35 (2D3)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for VPS35 Antibody (2D3)
![Western Blot: VPS35 Antibody (2D3) [H00055737-M02]](https://resources.bio-techne.com/images/products/VPS35-Antibody-2D3-Western-Blot-H00055737-M02-img0002.jpg "Western Blot: VPS35 Antibody (2D3) [H00055737-M02]")
Western Blot: VPS35 Antibody (2D3) [H00055737-M02]
Western Blot: VPS35 Antibody (2D3) [H00055737-M02] - VPS35 monoclonal antibody (M02), clone 2D3. Analysis of VPS35 expression in human colon.![Western Blot: VPS35 Antibody (2D3) [H00055737-M02]](https://resources.bio-techne.com/images/products/VPS35-Antibody-2D3-Western-Blot-H00055737-M02-img0001.jpg "Western Blot: VPS35 Antibody (2D3) [H00055737-M02]")
Western Blot: VPS35 Antibody (2D3) [H00055737-M02]
Western Blot: VPS35 Antibody (2D3) [H00055737-M02] - VPS35 monoclonal antibody (M02), clone 2D3. Analysis of VPS35 expression in HeLa.![ELISA: VPS35 Antibody (2D3) [H00055737-M02]](https://resources.bio-techne.com/images/products/VPS35-Antibody-2D3-ELISA-H00055737-M02-img0003.jpg "ELISA: VPS35 Antibody (2D3) [H00055737-M02]")
ELISA: VPS35 Antibody (2D3) [H00055737-M02]
ELISA: VPS35 Antibody (2D3) [H00055737-M02] - Detection limit for recombinant GST tagged VPS35 is 0.03 ng/ml as a capture antibody.Applications for VPS35 Antibody (2D3)
Application
Recommended Usage
ELISA
1:100-1:2000
Western Blot
1:500
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 28146471).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: VPS35
Alternate Names
DKFZp434P1672, FLJ10752, FLJ13588, FLJ20388, hVPS35, Maternal-embryonic 3, MEM3DKFZp434E1211, vacuolar protein sorting 35 (yeast homolog), vacuolar protein sorting 35 (yeast), vacuolar protein sorting 35 homolog (S. cerevisiae), vacuolar protein sorting-associated protein 35, Vesicle protein sorting 35
Entrez Gene IDs
55737 (Human)
Gene Symbol
VPS35
OMIM
606931 (Human)
UniProt
Additional VPS35 Products
Product Documents for VPS35 Antibody (2D3)
Product Specific Notices for VPS35 Antibody (2D3)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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