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VPS35 Antibody (8D3L3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16803

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-16803-100ul
NBP3-16803-20ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8D3L3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for VPS35 Antibody (8D3L3)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human VPS35 (Q96QK1). YAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VPS35 Antibody (8D3L3)

Western Blot: VPS35 Antibody (8D3L3) [NBP3-16803]

Western Blot: VPS35 Antibody (8D3L3) [NBP3-16803]

Western Blot: VPS35 Antibody (8D3L3) [NBP3-16803] - Western blot analysis of extracts of various cell lines, using VPS35 Rabbit mAb (NBP3-16803) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Applications for VPS35 Antibody (8D3L3)

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VPS35

The maintenance of organelles in eukaryotic cells depends on sorting proteins, which ensure the proper delivery of organelle-specific proteins. In S. cerevisiae, VPS35 has a direct role in the retrieval of vacuolar cargo proteins, suggesting that human VPS35 may have an analogous function in the maintenance of lysosomes.

Alternate Names

DKFZp434P1672, FLJ10752, FLJ13588, FLJ20388, hVPS35, Maternal-embryonic 3, MEM3DKFZp434E1211, vacuolar protein sorting 35 (yeast homolog), vacuolar protein sorting 35 (yeast), vacuolar protein sorting 35 homolog (S. cerevisiae), vacuolar protein sorting-associated protein 35, Vesicle protein sorting 35

Gene Symbol

VPS35

Additional VPS35 Products

Product Documents for VPS35 Antibody (8D3L3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VPS35 Antibody (8D3L3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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