VPS35 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1397

Novus Biologicals, part of Bio-Techne
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NB100-1397
NB100-1397-25ug
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Chicken, Chinese Hamster

Cited:

Human, Mouse

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, WB, IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all VPS35 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SPESEGPIYEGLIL corresponding to C-Terminus according to NP_060676.2.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Bovine. It has been reported in the literature to react with Chinese hamster ovary cells (PMID: 22747682). Mouse reactivity reported in scientific literature (PMID: 28252385). Canine, chicken and rat reactivity reported from verified customer reviews.

Specificity

Note there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VPS35 Antibody

Western Blot: VPS35 Antibody [NB100-1397]

Western Blot: VPS35 Antibody [NB100-1397]

Western Blot: VPS35 Antibody [NB100-1397] - Staining of HepG2 (A) and HEK293 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: VPS35 Antibody [NB100-1397]

Immunocytochemistry/ Immunofluorescence: VPS35 Antibody [NB100-1397]

Immunocytochemistry/Immunofluorescence: VPS35 Antibody [NB100-1397] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Paraffin: VPS35 Antibody [NB100-1397]

Immunohistochemistry-Paraffin: VPS35 Antibody [NB100-1397]

Immunohistochemistry-Paraffin: VPS35 Antibody [NB100-1397] - Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.
View 6 more images

Applications for VPS35 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

8 ug/mL

Immunohistochemistry-Paraffin

8 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.03 - 0.1 ug/mL
Application Notes
Western blot: Approx 90 kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates (calculated MW of 91.7 kDa according to NP_060676.2).

Reviewed Applications

Read 5 reviews rated 4.8 using NB100-1397 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VPS35

Chemokine receptor D6 expression has been documented in human skin, small intestine, large intestine, appendix, lymph node, vessels, tonsil, spleen, as well as from rat brain tissues. ESTs have been isolated from cancerous human lung, uterus, cervix, placenta, blood, and from normal human placenta libraries. Caution: G protein-coupled receptor D6 was previously called CCR10 and/or CCR9. CCR9 and C-C chemokine receptor 9 referred to both chemokine receptor D6 and the unrelated receptor GPR-9-6.

Alternate Names

DKFZp434P1672, FLJ10752, FLJ13588, FLJ20388, hVPS35, Maternal-embryonic 3, MEM3DKFZp434E1211, vacuolar protein sorting 35 (yeast homolog), vacuolar protein sorting 35 (yeast), vacuolar protein sorting 35 homolog (S. cerevisiae), vacuolar protein sorting-associated protein 35, Vesicle protein sorting 35

Entrez Gene IDs

55737 (Human); 65114 (Mouse)

Gene Symbol

VPS35

UniProt

NP_060676.2 (Human)

Additional VPS35 Products

Product Documents for VPS35 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VPS35 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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