VPS35 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13517

Novus Biologicals, part of Bio-Techne
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NBP2-13517
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VPS35 Antibodies »

Product Summary for VPS35 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: SCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VPS35 Antibody

Western Blot: VPS35 Antibody [NBP2-13517]

Western Blot: VPS35 Antibody [NBP2-13517]

Western Blot: VPS35 Antibody [NBP2-13517] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-VPS35 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunocytochemistry/ Immunofluorescence: VPS35 Antibody [NBP2-13517]

Immunocytochemistry/ Immunofluorescence: VPS35 Antibody [NBP2-13517]

Immunocytochemistry/Immunofluorescence: VPS35 Antibody [NBP2-13517] - Staining of human cell line U-2 OS shows localization to endosomes & lysosomes. Antibody staining is shown in green.
Western Blot: VPS35 Antibody [NBP2-13517]

Western Blot: VPS35 Antibody [NBP2-13517]

Western Blot: VPS35 Antibody [NBP2-13517] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for VPS35 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04 - 0.4 ug/ml
Application Notes
ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VPS35

The maintenance of organelles in eukaryotic cells depends on sorting proteins, which ensure the proper delivery of organelle-specific proteins. In S. cerevisiae, VPS35 has a direct role in the retrieval of vacuolar cargo proteins, suggesting that human VPS35 may have an analogous function in the maintenance of lysosomes.

Alternate Names

DKFZp434P1672, FLJ10752, FLJ13588, FLJ20388, hVPS35, Maternal-embryonic 3, MEM3DKFZp434E1211, vacuolar protein sorting 35 (yeast homolog), vacuolar protein sorting 35 (yeast), vacuolar protein sorting 35 homolog (S. cerevisiae), vacuolar protein sorting-associated protein 35, Vesicle protein sorting 35

Gene Symbol

VPS35

Additional VPS35 Products

Product Documents for VPS35 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VPS35 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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