VPS35 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20876

Novus Biologicals, part of Bio-Techne
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NBP2-20876
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Chicken (98%), Rat (100%), Xenopus (97%), Zebrafish (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VPS35 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human VPS35. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (97%).

Localization

Cytoplasm; Membrane; Peripheral membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VPS35 Antibody

Western Blot: VPS35 Antibody [NBP2-20876]

Western Blot: VPS35 Antibody [NBP2-20876]

Western Blot: VPS35 Antibody [NBP2-20876] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: VPS35 Antibody [NBP2-20876]

Immunohistochemistry-Paraffin: VPS35 Antibody [NBP2-20876]

Immunohistochemistry-Paraffin: VPS35 Antibody [NBP2-20876] - A549 xenograft, using antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min
Western Blot: VPS35 Antibody [NBP2-20876]

Western Blot: VPS35 Antibody [NBP2-20876]

Western Blot: VPS35 Antibody [NBP2-20876] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:1000.
View 2 more images

Applications for VPS35 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: VPS35

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq]

Alternate Names

DKFZp434P1672, FLJ10752, FLJ13588, FLJ20388, hVPS35, Maternal-embryonic 3, MEM3DKFZp434E1211, vacuolar protein sorting 35 (yeast homolog), vacuolar protein sorting 35 (yeast), vacuolar protein sorting 35 homolog (S. cerevisiae), vacuolar protein sorting-associated protein 35, Vesicle protein sorting 35

Gene Symbol

VPS35

UniProt

Q96QK1 (Human)

Additional VPS35 Products

Product Documents for VPS35 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VPS35 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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