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VPS37C Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2438

Novus Biologicals, part of Bio-Techne
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NB100-2438

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-ETLKDKTLQELEELQ corresponding to internal region according to NP_060436.4.

Epitope

C-ETLKDKTLQELEELQ

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for VPS37C Antibody

Western Blot: VPS37C Antibody [NB100-2438]

Western Blot: VPS37C Antibody [NB100-2438]

Western Blot: VPS37C Antibody [NB100-2438] - (0.03g/ml) staining of human duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: VPS37C Antibody [NB100-2438]

Immunohistochemistry-Paraffin: VPS37C Antibody [NB100-2438]

Immunohistochemistry-Paraffin: VPS37C Antibody [NB100-2438] - (3.8ug/ml) staining of Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for VPS37C Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 6 ug/ml

Immunohistochemistry-Paraffin

3-6 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.03 - 0.1 ug/ml
Application Notes
WB: Approx. 38 kDa band observed in human duodenum lysates (calculated MW of 38.7 kDa band according to NP_060436.4). IHC-P: Human prostate shows strong cytoplasm staining in stromal cells. Paraffin embedded Human Prostate and Breast recommended concentration: 3.75ug/ml.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VPS37C

VPS37C is a subunit of ESCRT-I (endosomal sorting complex required for transport I), a complex in the class E vacuolar protein sorting (VPS) pathway required for sorting ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies (Eastman et al., 2005).

Alternate Names

ESCRT-I complex subunit VPS37C, FLJ20847, hVps37C, PML39, vacuolar protein sorting 37 homolog C (S. cerevisiae), vacuolar protein sorting 37C, vacuolar protein sorting 37C (yeast), vacuolar protein sorting-associated protein 37C

Entrez Gene IDs

55048 (Human)

Gene Symbol

VPS37C

Additional VPS37C Products

Product Documents for VPS37C Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VPS37C Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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