VPS4B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20881

Novus Biologicals, part of Bio-Techne
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NBP2-20881
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (96%), Mouse (96%), Rat (96%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VPS4B Antibodies »

Product Summary for VPS4B Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human VPS4B. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (88%), Xenopus laevis (88%), Chicken (89%).

Localization

Prevacuolar compartment membrane; Peripheral membrane protein; Late endosome membrane; Peripheral membrane protein (Probable)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VPS4B Antibody

Western Blot: VPS4B Antibody [NBP2-20881]

Western Blot: VPS4B Antibody [NBP2-20881]

Western Blot: VPS4B Antibody [NBP2-20881] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:3000.
Immunocytochemistry/ Immunofluorescence: VPS4B Antibody [NBP2-20881]

Immunocytochemistry/ Immunofluorescence: VPS4B Antibody [NBP2-20881]

Immunocytochemistry/Immunofluorescence: VPS4B Antibody [NBP2-20881] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS4B protein stained by VPS4B antibody [N3C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: VPS4B Antibody [NBP2-20881]

Immunohistochemistry-Paraffin: VPS4B Antibody [NBP2-20881]

Immunohistochemistry-Paraffin: VPS4B Antibody [NBP2-20881] - Colon carcinoma. VPS4B antibody [N3C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min .
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Applications for VPS4B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: VPS4B

The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16. [provided by RefSeq]

Alternate Names

Cell migration-inducing gene 1 protein, Protein SKD1, SKD1B, SKD1cell migration-inducing 1, Suppressor of K(+) transport growth defect 1, suppressor of K+ transport defect 1, vacuolar protein sorting 4 homolog B (S. cerevisiae), vacuolar protein sorting 4B, vacuolar protein sorting 4B (yeast), vacuolar protein sorting-associated protein 4B, VPS42, VPS4-2

Gene Symbol

VPS4B

UniProt

O75351 (Human)

Additional VPS4B Products

Product Documents for VPS4B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VPS4B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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