VRL1 Antibody (HL1543) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25747

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25747
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1543

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VRL1 Antibodies »

Product Summary for VRL1 Antibody (HL1543) - Azide and BSA Free

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human VRL1. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VRL1 Antibody (HL1543) - Azide and BSA Free

VRL1 Antibody (HL1543) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: VRL1 Antibody (HL1543) - Azide and BSA Free [NBP3-25747] -

Immunocytochemistry/Immunofluorescence: VRL1 Antibody (HL1543) - Azide and BSA Free [NBP3-25747] - VRL1 antibody [HL1543] detects VRL1 protein at fiber by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VRL1 stained by VRL1 antibody [HL1543] (NBP3-25747) diluted at 1:250. Blue: Fluoroshield with DAPI .
VRL1 Antibody (HL1543) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: VRL1 Antibody (HL1543) - Azide and BSA Free [NBP3-25747] -

Immunocytochemistry/Immunofluorescence: VRL1 Antibody (HL1543) - Azide and BSA Free [NBP3-25747] - VRL1 antibody [HL1543] detects VRL1 protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VRL1 stained by VRL1 antibody [HL1543] (NBP3-25747) diluted at 1:500. Blue: Fluoroshield with DAPI .
VRL1 Antibody (HL1543) - Azide and BSA Free

Western Blot: VRL1 Antibody (HL1543) - Azide and BSA Free [NBP3-25747] -

Western Blot: VRL1 Antibody (HL1543) - Azide and BSA Free [NBP3-25747] - Unboiled K562 whole cell and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with VRL1 antibody [HL1543] (NBP3-25747) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (WCE: whole cell extract; ME: membrane extract)
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Applications for VRL1 Antibody (HL1543) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VRL1

Caterina et al has identified a structurally related receptor, VRL-1, that does not respond to capsaicin, acid or moderate heat. Instead, VRL-1 is activated by high temperatures, with a threshold of approximately 52 degrees C. Within sensory ganglia, VRL-1 is most prominently expressed by a subset of medium- to large-diameter neurons, making it a candidate receptor for transducing high-threshold heat responses in this class of cells. VRL-1 transcripts are not restricted to the sensory nervous system, indicating that this channel may be activated by stimuli other than heat. VRL-1 is one of the six members of the vanilloid-like TRP-channel family which is now known as TRPV family. FUNCTION: Calcium-permeable, non-selective cation channel with an outward rectification. Seems to be regulated, at least in part, by growth factors, like IGF1, PDGF and morphogenetic neuropeptide/head activator. May transduce physical stimuli in mast cells. Activated by temperatures higher than 52 degrees Celsius; is not activated by vanilloids and acidic pH. SUBUNIT: Homotetramer (Probable). Interacts with a cAMP-dependent protein kinase type II regulatory subunit (PRKAR2A or PRKAR2B) and ACBD3. Interacts with RGA. SUBCELLULAR LOCATION: Cell membrane; multi-pass membrane protein. Cytoplasm. Translocates from the cytoplasm to the plasma membrane upon ligand stimulation. TISSUE SPECIFICITY: Ubiquitously expressed. Expressed in dorsal root ganglia, trigeminal ganglia, spinal chord (Lissauer's tract, dorsal horn and dorsal columns) (at protein level). PTM: N-glycosylated. PTM: Phosphorylated by PKA. SIMILARITY: Belongs to the transient receptor family. TrpV subfamily. SIMILARITY: Contains 3 ANK repeats.

Long Name

Transient receptor potential cation channel subfamily V member 2

Alternate Names

OTRPC2, TrpV2, VRL, VRL-1

Gene Symbol

TRPV2

Additional VRL1 Products

Product Documents for VRL1 Antibody (HL1543) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for VRL1 Antibody (HL1543) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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