VSV-G Epitope Tag Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-95261
Key Product Details
Species Reactivity
Epitope Tag
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This affinity purified VSV-G Epitope Tag Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 501-511 of VSV-G Epitope Tag.
Reactivity Notes
Vesicular stomatitis virus (VSV)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against VSV-G Epitope Tag protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
This affinity purified antibody is directed against VSV-G Epitope Tag protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
Scientific Data Images for VSV-G Epitope Tag Antibody
![Western Blot: VSV-G Epitope Tag Antibody [NBP2-95261]](https://resources.bio-techne.com/images/products/VSV-G Epitope Tag Antibody-Western Blot-NBP2-95261-img0002.jpg "Western Blot: VSV-G Epitope Tag Antibody [NBP2-95261]")
Western Blot: VSV-G Epitope Tag Antibody [NBP2-95261]
Western Blot: VSV-G Epitope Tag Antibody [NBP2-95261] - Lane 1: 12 Epitope Tag Protein Marker Lysate.Lane 2: none. Load: ~10 ug per lane. Primary antibody: VSV-G Epitope Tag Antibody at 1:1,000 for overnight at 4C. Secondary antibody: DyLight(TM) 649 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 3% BSA-TBS 2H at RT. Predicted/Observed size: ~50 kDa for VSV-G. Other band(s): VSV-G splice variants and isoforms.Applications for VSV-G Epitope Tag Antibody
Application
Recommended Usage
ELISA
1:50000-1:100000
Immunocytochemistry/ Immunofluorescence
1:200-1:500
Immunohistochemistry
1:200-1:500
Western Blot
1:1000-1:5000
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blot. This product is suitable for immunofluorescence microscopy. Specific conditions for reactivity should be optimized by the end user. For standard indirect immunofluorescence assay we recommend paraformaldehyde fixation with detergent permeabilization of cells infected with VSV or transfected with the wild-type VSV-G protein. Staining is quite clean and specific for both sources of the G protein when diluted at least 1/200. At higher concentrations, a filamentous background staining may be present.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: VSV-G Epitope Tag
Alternate Names
Vesicular stomatitis virus glycoprotein tag, VSV epitope tag, VSV tag, VSVG, VSV-G epitope tag, vsvg tag
Additional VSV-G Epitope Tag Products
Product Documents for VSV-G Epitope Tag Antibody
Product Specific Notices for VSV-G Epitope Tag Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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