Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all WDR5 Antibodies »

Product Specifications

Immunogen

maps to a region between residue 1 and 50 of human WD repeat domain 5 using the numbering given in entry NP_060058.1

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WDR5 Antibody - BSA Free

Immunohistochemistry-Paraffin: WDR5 Antibody [NBP1-30063]

Immunohistochemistry-Paraffin: WDR5 Antibody [NBP1-30063]

Immunohistochemistry-Paraffin: WDR5 Antibody [NBP1-30063] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-fs18 WDR5 fs20 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: WDR5 Antibody [NBP1-30063]

Immunoprecipitation: WDR5 Antibody [NBP1-30063]

Immunoprecipitation: WDR5 Antibody [NBP1-30063] - Detection of human WDR5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-WDR5 antibody A302-429A (lot A302-429A-2) used for IP at 6 ug per reaction. WDR5 was also immunoprecipitated by rabbit anti-WDR5 antibody A302-430A. For blotting immunoprecipitated WDR5, A302-430A was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for WDR5 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2 - 10 ug/mg lysate
Application Notes
Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections. Western Blot Not recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: WDR5

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 7 WD repeats. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript. The two variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Long Name

WD Repeat-containing Protein 5

Alternate Names

BIG3, SWD3

Entrez Gene IDs

11091 (Human)

Gene Symbol

WDR5

UniProt

P61964

Additional WDR5 Products

Product Documents for WDR5 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WDR5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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