WDR5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30070

Novus Biologicals, part of Bio-Techne
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NBP1-30070
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation (Negative), Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all WDR5 Antibodies »

Product Summary for WDR5 Antibody

Immunogen

maps to a region between residue 50 and 100 of human WD repeat domain 5 using the numbering given in entry NP_060058.1

Reactivity Notes

D. melanogaster, X. tropicalis (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WDR5 Antibody

Western Blot: WDR5 Antibody [NBP1-30070]

Western Blot: WDR5 Antibody [NBP1-30070]

Western Blot: WDR5 Antibody [NBP1-30070] - Detection of mouse and rat WDR5 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-WDR5 antibody NBP1-30070 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: WDR5 Antibody [NBP1-30070]

Immunohistochemistry-Paraffin: WDR5 Antibody [NBP1-30070]

Immunohistochemistry-Paraffin: WDR5 Antibody [NBP1-30070] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- WDR5 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: WDR5 Antibody [NBP1-30070]

Western Blot: WDR5 Antibody [NBP1-30070]

Western Blot: WDR5 Antibody [NBP1-30070] - Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells. WDR5 was successfully IPed by NBP1-30063.

Applications for WDR5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:2000-1:10000
Application Notes
IP Not recommended. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
See Simple Western Antibody Database for Simple Western validation: tested in Human Cancer Cell Line; antibody dilution of 1:10; separated by size; detects a band at 47 kDa
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: WDR5

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 7 WD repeats. Alternatively spliced transcript variants encoding the same protein have been identified.

Long Name

WD Repeat-containing Protein 5

Alternate Names

BIG3, SWD3

Gene Symbol

WDR5

Additional WDR5 Products

Product Documents for WDR5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for WDR5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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