WDR5 Antibody

Western Blot: WDR5 Antibody [NBP3-18669]

Western Blot: WDR5 Antibody [NBP3-18669] - Figure 3. Western blot analysis using the antibody directed against WDR5 Nclear extracts from HeLa cells (20 ug) were analysed by Western blot using the antibody against WDR5 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Immunocytochemistry/ Immunofluorescence: WDR5 Antibody [NBP3-18669]

Immunocytochemistry/Immunofluorescence: WDR5 Antibody [NBP3-18669] - Figure 4. Immunofluorescence using the antibody directed against WDR HeLa cells were stained with the antibody against WDR5 and with DAPI. Cells were fixed with 4% formaldehyde for 10 and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the WDR5 antibody (left) diluted 1:1,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Chromatin Immunoprecipitation (ChIP): WDR5 Antibody [NBP3-18669] - Figure 1. ChIP results obtained with antibody directed against WDR5. ChIP assays performed using HeLa cells, antibody against WDR5 and optimized PCR primer sets for qPCR. ChIP performed with the "iDeal ChIP-seq" kit , using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody/ChIP experiment analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR performed with optimized primers for the promoters of the RPL41 and RPS4X ribosomal protein genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).