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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4C4

Concentration

1 mg/ml

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 92-305 of human WDR61(NP_079510) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

33.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for WDR61 Antibody (OTI4C4)

Western Blot: WDR61 Antibody (OTI4C4) [NBP2-46359]

Western Blot: WDR61 Antibody (OTI4C4) [NBP2-46359]

Western Blot: WDR61 Antibody (4C4) [NBP2-46359] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WDR61.
Immunohistochemistry: WDR61 Antibody (OTI4C4) [NBP2-46359]

Immunohistochemistry: WDR61 Antibody (OTI4C4) [NBP2-46359]

Immunohistochemistry: WDR61 Antibody (4C4) [NBP2-46359] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Western Blot: WDR61 Antibody (OTI4C4) [NBP2-46359]

Western Blot: WDR61 Antibody (OTI4C4) [NBP2-46359]

Western Blot: WDR61 Antibody (4C4) [NBP2-46359] - Analysis of extracts (10ug) from 5 different cell lines and 4 human tissue (1: Hela; 2: HepG2; 3: K562; 4: Mcf7; 5: SVT2; 6: Liver; 7: Testis; 8: Brain; 9: Uterus)

Applications for WDR61 Antibody (OTI4C4)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: WDR61

WDR61 is a subunit of the human PAF and SKI complexes, which function in transcriptional regulation and are involved in events downstream of RNA synthesis, such as RNA surveillance (Zhu et al., 2005 [PubMed 16024656]).[supplied by OMIM]

Alternate Names

Meiotic recombination REC14 protein homolog, REC14, recombination protein REC14, SKI8, WD repeat domain 61, WD repeat-containing protein 61

Gene Symbol

SKIC8

Additional WDR61 Products

Product Documents for WDR61 Antibody (OTI4C4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WDR61 Antibody (OTI4C4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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