WDR74 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84623

Novus Biologicals, part of Bio-Techne
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NBP1-84623
NBP1-84623-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all WDR74 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GGEGMFRGLAQADGTLITCVDSGILRVWHDKDKDTSSDPLLELRVGPGVCRMRQDPAHPHVVATGGKENALKIWDL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WDR74 Antibody

Western Blot: WDR74 Antibody [NBP1-84623]

Western Blot: WDR74 Antibody [NBP1-84623]

Western Blot: WDR74 Antibody [NBP1-84623] - Analysis in control (vector only transfected HEK293T lysate) and WDR74 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: WDR74 Antibody [NBP1-84623]

Immunohistochemistry-Paraffin: WDR74 Antibody [NBP1-84623]

Immunohistochemistry-Paraffin: WDR74 Antibody [NBP1-84623] - staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
Western Blot: WDR74 Antibody [NBP1-84623]

Western Blot: WDR74 Antibody [NBP1-84623]

Western Blot: WDR74 Antibody [NBP1-84623] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-637

Applications for WDR74 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WDR74

WDR74, also known as WD repeat-containing protein 74, has a 385 amino acid long isoform that is 42 kDa and a short 366 amino acid isoform that is approx. 40 kDa, is located in the nucleolus and known to be required for RNA transcription, processing and/or stability during pre-implantation development and blastocyst formation in mouse. It is associated with Jervell and Lange-Nielsen syndrome, Jacobsen syndrome and Niemann-Pick disease. This protein has also shown to have an interaction with RGS2, CALR and NUDT3 in blastocyst formation, biological and RNA metabolic processes.

Alternate Names

FLJ10439, FLJ21730, NOP seven-associated protein 1, NSA1, WD repeat domain 74, WD repeat-containing protein 74

Gene Symbol

WDR74

Additional WDR74 Products

Product Documents for WDR74 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for WDR74 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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