WDR74 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38411

Novus Biologicals, part of Bio-Techne
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NBP2-38411
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all WDR74 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: RNDWLDLRVPIWDQDIQFLPGSQKLVTCTGYHQVRVYDPASPQRRPVLETTYGEYPLTAMTLTPGGNSVIVGNTHGQLAEID

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WDR74 Antibody

Western Blot: WDR74 Antibody [NBP2-38411]

Western Blot: WDR74 Antibody [NBP2-38411]

Western Blot: WDR74 Antibody [NBP2-38411] - Analysis in control (vector only transfected HEK293T lysate) and WDR74 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: WDR74 Antibody [NBP2-38411]

Immunocytochemistry/ Immunofluorescence: WDR74 Antibody [NBP2-38411]

Immunocytochemistry/Immunofluorescence: WDR74 Antibody [NBP2-38411] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus & nucleoli.
Immunohistochemistry: WDR74 Antibody [NBP2-38411]

Immunohistochemistry: WDR74 Antibody [NBP2-38411]

Immunohistochemistry: WDR74 Antibody [NBP2-38411] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

Applications for WDR74 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WDR74

WDR74, also known as WD repeat-containing protein 74, has a 385 amino acid long isoform that is 42 kDa and a short 366 amino acid isoform that is approx. 40 kDa, is located in the nucleolus and known to be required for RNA transcription, processing and/or stability during pre-implantation development and blastocyst formation in mouse. It is associated with Jervell and Lange-Nielsen syndrome, Jacobsen syndrome and Niemann-Pick disease. This protein has also shown to have an interaction with RGS2, CALR and NUDT3 in blastocyst formation, biological and RNA metabolic processes.

Alternate Names

FLJ10439, FLJ21730, NOP seven-associated protein 1, NSA1, WD repeat domain 74, WD repeat-containing protein 74

Gene Symbol

WDR74

UniProt

Q6RFH5 (Human)

Additional WDR74 Products

Product Documents for WDR74 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for WDR74 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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