WDR77 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82778

Novus Biologicals, part of Bio-Techne
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NBP1-82778
NBP1-82778-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all WDR77 Antibodies »

Product Summary for WDR77 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SEDCSIAVLDSSLSELFRSQAHRDFVRDATWSPLNHSLLTTVGWDHQVVHHVVPTEPLPAPGPASVTE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WDR77 Antibody

Western Blot: WDR77 Antibody [NBP1-82778]

Western Blot: WDR77 Antibody [NBP1-82778]

Western Blot: WDR77 Antibody [NBP1-82778] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: WDR77 Antibody [NBP1-82778]

Immunocytochemistry/ Immunofluorescence: WDR77 Antibody [NBP1-82778]

Immunocytochemistry/Immunofluorescence: WDR77 Antibody [NBP1-82778] - Staining of human cell line A-431 shows localization to nucleoplasm, cytosol & the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: WDR77 Antibody [NBP1-82778]

Immunohistochemistry-Paraffin: WDR77 Antibody [NBP1-82778]

Immunohistochemistry-Paraffin: WDR77 Antibody [NBP1-82778] - Staining of human rectum shows weak to moderate cytoplasmic and nuclear positivity in glandular cells.
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Applications for WDR77 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WDR77

WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061). This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al., 2002 [PubMed 11756452]).[supplied by OMIM]

Alternate Names

Androgen receptor cofactor p44, MEP-50, MEP50Nbla10071, methylosome protein 50, MGC2722, p44, p44/Mep50, RP11-552M11.3, WD repeat domain 77, WD repeat-containing protein 77

Gene Symbol

WDR77

Additional WDR77 Products

Product Documents for WDR77 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for WDR77 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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