WDR77 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98916

Novus Biologicals, part of Bio-Techne
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NBP2-98916-100ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all WDR77 Antibodies »

Product Summary for WDR77 Antibody

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human WDR77.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for WDR77 Antibody

Western Blot: WDR77 Antibody [NBP2-98916]

Western Blot: WDR77 Antibody [NBP2-98916]

Western Blot: WDR77 Antibody [NBP2-98916] - Anti-WDR77 rabbit polyclonal antibody at 1:500 dilution. Lane A: PC12 Whole Cell Lysate Lane B: NIH3T3 Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 37 kDa. Observed band size: 40 kDa (We are unsure as to the identity of these extra bands).
Immunocytochemistry/ Immunofluorescence: WDR77 Antibody [NBP2-98916]

Immunocytochemistry/ Immunofluorescence: WDR77 Antibody [NBP2-98916]

Immunocytochemistry/Immunofluorescence: WDR77 Antibody [NBP2-98916] - Immunofluorescence staining of WDR77 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human WDR77 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.
Immunohistochemistry-Paraffin: WDR77 Antibody [NBP2-98916]

Immunohistochemistry-Paraffin: WDR77 Antibody [NBP2-98916]

Immunohistochemistry-Paraffin: WDR77 Antibody [NBP2-98916] - Immunochemical staining of human WDR77 in human testis with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
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Applications for WDR77 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry-Paraffin

1:500-1:2000

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WDR77

WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061). This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al., 2002 [PubMed 11756452]).[supplied by OMIM]

Alternate Names

Androgen receptor cofactor p44, MEP-50, MEP50Nbla10071, methylosome protein 50, MGC2722, p44, p44/Mep50, RP11-552M11.3, WD repeat domain 77, WD repeat-containing protein 77

Gene Symbol

WDR77

Additional WDR77 Products

Product Documents for WDR77 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for WDR77 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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